Shh RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:55332 Takeda S, et al., Left-right asymmetry and kinesin superfamily protein KIF3A: new insights in determination of laterality and mesoderm induction by kif3A-/- mice analysis. J Cell Biol. 1999 May 17;145(4):825-36
Assay type:	RNA in situ
MGI Accession ID:	MGI:6113405
Gene symbol:	Shh
Gene name:	sonic hedgehog

Probe:	Shh probe131
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

10E upper ©

Specimen 10E upper: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: notochord	Present		10E upper
TS13: future midbrain	Present	Regionally restricted	10E upper	Expressed in the ventral half.

10E lower ©

Specimen 10E lower: embryonic day 8.5; Kif3a^tm1Noh/Kif3a^tm1Noh (more

)

Structure	Level	Pattern	Image	Note
TS13: notochord	Present		10E lower	Significantly reduced expression compared with the wild-type.
TS13: future midbrain	Absent		10E lower

10F upper ©

Specimen 10F upper: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: notochord	Present		10F upper
TS13: future midbrain	Present	Regionally restricted	10F upper	Expressed in the ventral half.

10F lower ©

Specimen 10F lower: embryonic day 8.5; Kif3a^tm1Noh/Kif3a^tm1Noh (more

)

Structure	Level	Pattern	Image	Note
TS13: notochord	Present		10F lower	Significantly reduced expression compared with the wild-type.
TS13: future midbrain	Absent		10F lower

10G left ©

Specimen 10G left: embryonic day 7.5 (more

)

Structure	Level	Pattern	Image	Note
TS11: node	Strong		10G left
TS11: embryo mesoderm	Present	Regionally restricted	10G left	Expressed in the midline mesoderm.

10G middle ©

Specimen 10G middle: embryonic day 7.5; Kif3a^tm1Noh/Kif3a^tm1Noh (more

)

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present		10G middle	Dramatically reduced expression without strong localization within node compared with wild-type.
TS11: node	Present		10G middle	Dramatically reduced expression without strong localization within node compared with wild-type.

10G right ©

Specimen 10G right: embryonic day 7.5 (more

)

Structure	Level	Pattern	Image	Note
TS11: node	Strong		10G right
TS11: embryo mesoderm	Present	Regionally restricted	10G right	Expressed in the midline mesoderm.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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