Antibody:	anti-CAR
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present	Regionally restricted	9_CAR 4 dpc	Expression is seen in the cell contacts.
TS4: mural trophectoderm	Present	Regionally restricted	9_CAR 4 dpc	Expression is seen in the cell surface and cell contacts.
TS4: polar trophectoderm	Present	Regionally restricted	9_CAR 4 dpc	Expression is seen in the cell surface and cell contacts.

Structure	Level	Pattern	Image	Note
TS28: endometrium epithelium	Present	Regionally restricted	9_CAR 4 dpc	Expression is seen in the apicolateral contacts between adjacent luminal epithelial cells.

Structure	Level	Pattern	Image	Note
TS5: blastocyst	Present		9_CAR 4.5 dpc

Structure	Level	Pattern	Image	Note
TS28: endometrium epithelium	Present	Regionally restricted	9_CAR 4.5 dpc	Expression is seen in the apicolateral contacts between adjacent luminal epithelial cells.

Structure	Level	Pattern	Image	Note
TS6: epiblast	Strong	Non-Uniform	9_CAR 5 dpc	Expression is strong in the inner core but weak in surrounding cells.
TS6: primitive endoderm	Present		9_CAR 5 dpc
TS6: extraembryonic component	Present		9_CAR 5 dpc	Expression was seen in the extraembryonic ectoderm.

Structure	Level	Pattern	Image	Note
TS28: endometrium epithelium	Present	Regionally restricted	9_CAR 5 dpc	Expression is seen in the apicolateral contacts between adjacent luminal epithelial cells.

Structure	Level	Pattern	Image	Note
TS7: epiblast	Strong	Non-Uniform	9_CAR 6 dpc	Expression is seen in the apical contacts between adjacent inner cells surrounding the developing pro-amniotic cavity.
TS7: primitive endoderm	Present	Regionally restricted	9_CAR 6 dpc	Expression is found in the apicolateral contacts between adjacent visceral endoderm cells.