Antibody:	anti-CAR
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage embryo	Present		5_4-cell	Expression is seen in the contacts between blastomeres and diffusely in the cytoplasm.

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present		5_8-cell	Expression is seen in the contacts between blastomeres and diffusely in the cytoplasm.

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present		5_Morula	Expression is seen in the lateral contacts between blastomeres; there is a decrease in cytoplasmic signal.

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		5_Nascent blastocyst	Expression is found in the lateral contacts contacts between blastomeres.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		5_Expanded blastocyst	Expression is found at the apicolateral contacts between cells.
TS4: trophectoderm	Present		5_Expanded blastocyst	Expression is found at the apicolateral contacts between cells.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		6_CAR , 6_Merged 3D , 6_Merged 2D	Expression is seen at the apical contacts between cells, as well as lateral contacts.
TS4: trophectoderm	Present		6_CAR , 6_Merged 3D , 6_Merged 2D	Expression is seen at the apical contacts between cells, as well as lateral contacts.