Probe:	Ebf cDNA2
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS18: neural tube mantle layer	Present	Regionally restricted	3E	Expression is in the subventricular mantle layer and is absent in a few patches.
TS18: neural tube basal columns	Weak		3E

Structure	Level	Pattern	Image	Note
TS19: neural tube mantle layer	Strong		3H

Structure	Level	Pattern	Image	Note
TS19: hindbrain mantle layer	Present	Regionally restricted	4D	Expression is caudal.
TS19: hindbrain ventricular layer	Present	Regionally restricted	4D	Expression is in the rostral-most hindbrain.
TS19: midbrain-hindbrain junction	Absent		4D

Structure	Level	Pattern	Image	Note
TS19: hindbrain mantle layer	Present		4G
TS19: hindbrain ventricular layer	Present	Single cells	4G	Expression is in a few cells.

Structure	Level	Pattern	Image	Note
TS22: pons	Present	Regionally restricted	4K	Expression is in the facial nucleus and superior olive.
TS22: cerebellum gray matter	Present	Regionally restricted	4K	Expression is in the cortical transitory zone.
TS22: deep cerebellar nucleus	Absent		4K

Structure	Level	Pattern	Image	Note
TS21: midbrain	Present	Widespread	5A
TS21: forebrain-midbrain boundary region	Strong	Regionally restricted	5A	Expression is in the precommissural zone.
TS21: thalamus	Present	Regionally restricted	5A	Expression is in the dorsal thalamus and is absent in the ventral thalamus.
TS21: posterior commissure	Present		5A
TS21: cerebral cortex	Present	Regionally restricted	5A	Expression is in the external layer.

Structure	Level	Pattern	Image	Note
TS21: mammillary area	Present		5D
TS21: forebrain	Present	Regionally restricted	5D	Expression is in the eminentia thalami.
TS21: cerebral cortex	Weak	Regionally restricted	5D	Expression is in the external layer.
TS21: zona limitans intrathalamica	Weak		5D

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present		6A
TS20: posterior commissure	Present		6A
TS20: forebrain-midbrain boundary region	Present	Regionally restricted	6A	Expression is in the pretectum.
TS20: thalamus	Present	Regionally restricted	6A	Expression is in the ventral thalamus.
TS20: medial ganglionic eminence	Present	Regionally restricted	6A	Rostrally, expression is in part of the medial ganglionic eminence.
TS20: lateral ganglionic eminence mantle zone	Present		6A
TS20: telencephalon	Present	Regionally restricted	6A	Expression is in a thin external layer of the septum.

Structure	Level	Pattern	Image	Note
TS20: forebrain-midbrain boundary region	Strong	Regionally restricted	6C	Expression is in the pretectum in the precommissural zone.
TS20: thalamus	Present	Graded	6C	Expression is in the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence mantle zone	Present		6E
TS21: cerebral cortex	Present	Regionally restricted	6E	Expression is in layer I of the cortex.
TS21: forebrain	Present	Regionally restricted	6E	Expression is in the hippocampus.
TS21: main olfactory bulb	Present		6E
TS21: neural retina	Present		6E

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	6G	Expression is in the basal ganglia and in a thin external layer of the septum.
TS20: lateral ganglionic eminence	Present		6G
TS20: telencephalon mantle layer	Present		6G

Structure	Level	Pattern	Image	Note
TS15: future midbrain lateral wall	Present	Single cells		Expression is in a few cells in the alar plate.
TS15: diencephalon	Present	Regionally restricted		Expression is in the alar and basal plates of the caudal part of the diencephalon.

Structure	Level	Pattern	Image	Note
TS16: future midbrain	Present	Graded		There is a rostrocaudal decreasing gradient of expression.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted		Expression is absent in a longitudinal band at the level of the hypothalamic cell cord.

Structure	Level	Pattern	Image	Note
TS21: dorsal grey horn	Present
TS21: spinal cord intermediate grey	Present
TS21: midbrain	Present	Regionally restricted		From E13.5 there is a progressive down-regulation of expression in the basal plate, while expression was maintained in the tectum.
TS21: basal columns	Absent

Structure	Level	Pattern	Image	Note
TS22: corpus striatum	Present
TS22: hippocampus	Present
TS22: pretectum	Present
TS22: spinal cord dorsal horn	Present
TS22: spinal cord intermediate grey	Present
TS22: pons	Present	Regionally restricted		Expression is in the cochlear nucleus.
TS22: cerebral cortex	Present	Regionally restricted		Expression is in the external layer.
TS22: midbrain	Present	Regionally restricted		Expression is downregulated in the basal plate.