Pou5f1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:237226 Ghule PN, et al., Maternal expression and early induction of histone gene transcription factor Hinfp sustains development in pre-implantation embryos. Dev Biol. 2016 Nov 15;419(2):311-320
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5912254
Gene symbol:	Pou5f1
Gene name:	POU domain, class 5, transcription factor 1

Antibody:	anti-OCT4
Detection system:	Secondary antibody

Results

2B WT Merge ©

2B WT Oct4 ©

Specimen 2B WT: embryonic day 3.5 (more

)
Note: Double labeled: red - Pou5f1; green Cdx2 (assay MGI:5912255).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		2B WT Oct4 , 2B WT Merge

2B HET Merge ©

2B HET Oct4 ©

Specimen 2B HET: embryonic day 3.5; Hinfp^tm1Gss/Hinfp⁺ (more

)
Note: Double labeled: red - Pou5f1; green Cdx2 (assay MGI:5912255).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		2B HET Oct4 , 2B HET Merge

2B KO Merge ©

2B KO Oct4 ©

Specimen 2B KO: embryonic day 3.5; Hinfp^tm1Gss/Hinfp^tm1Gss (more

)
Note: Double labeled: red - Pou5f1; green Cdx2 (assay MGI:5912255).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		2B KO Oct4 , 2B KO Merge

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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