About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:243676 Wen J, et al., Single-cell analysis reveals lineage segregation in early post-implantation mouse embryos. J Biol Chem. 2017 Jun 09;292(23):9840-9854
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5912161
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct4 (N124)
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 3B early: embryonic day 6.5 (more )
Note: Double labeled: red - Pou5f1; green - Gata6 (assay MGI:5912159)
Structure Level Pattern Image Note
TS9: embryo Present 3B merge left , 3B Oct4 left Expression is seen in the epiblast.
TS9: primitive streak Present 3B Oct4 left , 3B merge left
TS9: primitive endoderm Absent 3B merge left , 3B Oct4 left No expression is seen in the visceral endoderm.
Specimen 3B late: embryonic day 6.5 (more )
Note: Double labeled: red - Pou5f1; green - Gata6 (assay MGI:5912159)
Structure Level Pattern Image Note
TS10: embryo Present 3B merge center , 3B Oct4 center Expression is seen in the epiblast.
TS10: primitive streak Present 3B merge center , 3B Oct4 center , 3B merge right , 3B Oct4 right
TS10: primitive endoderm Absent 3B Oct4 center , 3B merge center No expression is seen in the visceral endoderm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory