Hoxb8 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:19897 Charite J, et al., Ectopic expression of Hoxb-8 causes duplication of the ZPA in the forelimb and homeotic transformation of axial structures. Cell. 1994 Aug 26;78(4):589-601
Assay type:	RNA in situ
MGI Accession ID:	MGI:5910909
Gene symbol:	Hoxb8
Gene name:	homeobox B8

Probe:	Hox 2.4 probe4
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 1c: embryonic day 9.0 (more

)
Note: 13 somites.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	1c	Posterior expression with an anterior boundary of expression at the level of the 5th somite.
TS14: somite 10	Present	Regionally restricted	1c	The anterior boundary of expression is at somite 10-11.
TS14: somite 11	Present	Regionally restricted	1c	The anterior boundary of expression is at somite 10-11.
TS14: mesenchyme derived from lateral plate	Present	Regionally restricted	1c	Expression is posterior somite 10-11.
TS14: trunk somite	Present	Regionally restricted	1c	Expression is posterior somite 10-11.
TS14: trunk unsegmented mesenchyme	Present	Regionally restricted	1c	Expression is posterior somite 10-11.
TS14: unsegmented mesenchyme	Present	Regionally restricted	1c	Expression is posterior somite 10-11.

Specimen 4a: embryonic day 9.5 (more

)
Note: 22 somites.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present	Regionally restricted	4a	Expression is in the posterior part.

Specimen 4b: embryonic day 9.5 (more

)
Note: 25 somites.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present	Regionally restricted	4b	Expression is in the posterior part.

Specimen 4c: embryonic day 9.5 (more

)
Note: 29 somites.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Weak		4c

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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