Probe:	Fstl1 cDNA2
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS10: embryo	Present		2B
TS10: extraembryonic component	Absent		2B

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Present		2B inset
TS10: embryo endoderm	Present		2B inset
TS10: embryo mesoderm	Present		2B inset

Structure	Level	Pattern	Image	Note
TS15: embryo	Weak	Ubiquitous	2C

Structure	Level	Pattern	Image	Note
TS15: future forebrain floor plate	Present		2D
TS15: future forebrain roof plate	Present	Regionally restricted	2D
TS15: optic vesicle	Absent		2D

Structure	Level	Pattern	Image	Note
TS15: blood vessel	Present		2E
TS15: neural tube floor plate	Present		2E
TS15: optic vesicle	Absent		2E

Structure	Level	Pattern	Image	Note
TS15: dermomyotome	Strong		2F
TS15: nephric duct	Strong		2F
TS15: somite	Present		2F

Structure	Level	Pattern	Image	Note
TS15: heart	Present	Ubiquitous	2G

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Present		2H

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Regionally restricted	3A	Expression is in the majority of the embryo with the exception of liver, most of the central nervous system, and peripheral nervous system.
TS20: central nervous system	Present	Regionally restricted	3A
TS20: lung	Present		3A
TS20: liver	Absent		3A
TS20: peripheral nervous system	Absent		3A

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Regionally restricted	3B	Expression is in the majority of the embryo with the exception of liver, most of the central nervous system, and peripheral nervous system.
TS20: central nervous system	Present	Regionally restricted	3B
TS20: heart	Present	Regionally restricted	3B
TS20: lung	Present		3B
TS20: metanephros	Present		3B
TS20: liver	Absent		3B
TS20: peripheral nervous system	Absent		3B

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Regionally restricted	3C	Expression is in the majority of the embryo with the exception of liver, most of the central nervous system, and peripheral nervous system.
TS20: blood vessel	Present		3C
TS20: central nervous system	Present	Regionally restricted	3C
TS20: heart	Present	Regionally restricted	3C
TS20: liver	Absent		3C
TS20: peripheral nervous system	Absent		3C

Structure	Level	Pattern	Image	Note
TS20: brain floor plate	Present		3D

Structure	Level	Pattern	Image	Note
TS20: hindbrain floor plate	Present		3E

Structure	Level	Pattern	Image	Note
TS20: choroid plexus	Present		3F

Structure	Level	Pattern	Image	Note
TS20: optic stalk	Strong		3G
TS20: pigmented retinal epithelium	Strong		3G
TS20: lens vesicle	Present	Regionally restricted	3G	Expression is largely absent.
TS20: neural retina	Present	Regionally restricted	3G	Expression is largely absent.

Structure	Level	Pattern	Image	Note
TS20: inner ear mesenchyme	Strong		3H
TS20: inner ear epithelium	Weak		3H

Structure	Level	Pattern	Image	Note
TS20: nasal mesenchyme	Strong		3I
TS20: olfactory epithelium	Absent		3I

Structure	Level	Pattern	Image	Note
TS20: trunk mesenchyme	Present	Regionally restricted	3J	Expression is in the mesenchyme surrounding the dorsal root ganglia.
TS20: dorsal root ganglion	Absent		3J
TS20: vertebral cartilage condensation	Absent		3J

Structure	Level	Pattern	Image	Note
TS20: limb mesenchyme	Strong		3K
TS20: limb digit	Absent		3K
TS20: limb pre-cartilage condensation	Absent		3K

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Strong	Regionally restricted	3L	Expression is in the mesenchyme surrounding the long bones.
TS20: radius-ulna pre-cartilage condensation	Absent		3L

Structure	Level	Pattern	Image	Note
TS20: outflow tract	Strong		4A
TS20: heart atrium	Weak		4A	There is a low-level generalized expression.
TS20: heart ventricle	Weak		4A	There is a low-level generalized expression.

Structure	Level	Pattern	Image	Note
TS20: dorsal aorta	Present	Regionally restricted	4B	Expression is in the vessel wall in smooth muscle cells.
TS20: smooth muscle tissue	Present	Regionally restricted	4B	Expression is in the vessel wall in smooth muscle cells.
TS20: blood vessel endothelium	Present		4B

Structure	Level	Pattern	Image	Note
TS20: dorsal aorta	Present	Regionally restricted	4C	Expression is in the vessel wall in smooth muscle cells.
TS20: smooth muscle tissue	Present	Regionally restricted	4C	Expression is in the vessel wall in smooth muscle cells.
TS20: blood vessel endothelium	Present		4C

Structure	Level	Pattern	Image	Note
TS20: gut mesenchyme	Present		5A	Expression is highest in the sub-epithelial vascular presumptive lamina propria and in the mesothelium surrounding the gut, and low or absent in developing smooth muscle.
TS20: gut epithelium	Absent		5A

Structure	Level	Pattern	Image	Note
TS22: lung mesenchyme	Strong	Regionally restricted	6A	Expression is in the mesenchyme surrounding the larger airways.
TS22: smooth muscle tissue	Strong	Regionally restricted	6A	Expression is within the smooth muscle layer surrounding airways.
TS22: lung vascular element	Strong	Regionally restricted	6A	Expression is in the walls of the blood vessels.
TS22: lung	Present		6A	There is generalized expression in the lung.

Structure	Level	Pattern	Image	Note
TS25: blood vessel endothelium	Present	Regionally restricted	6E	Expression is in the lung endothelium.
TS25: lung vascular element	Present	Regionally restricted	6E	Expression is in the lung endothelium.
TS25: lung epithelium	Present	Regionally restricted	6E inset , 6E	Expression is in Goblet cells.
TS25: smooth muscle tissue	Weak	Regionally restricted	6E	Expression is in in airway smooth muscle.

Structure	Level	Pattern	Image	Note
TS22: collecting duct	Strong		7A'
TS22: early nephron	Strong		7A'
TS22: ureteric tip	Strong		7A'
TS22: metanephros	Weak		7A'	There is weak and generalized expression.
TS22: mesenchyme of metanephros	Absent		7A'
TS22: ureteric trunk	Absent		7A'

Structure	Level	Pattern	Image	Note
TS22: collecting duct	Strong		7B'
TS22: ureteric tip	Strong		7B'
TS22: metanephros	Weak		7B'	There is weak and generalized expression.
TS22: mesenchyme of metanephros	Absent		7B'
TS22: ureteric trunk	Absent		7B'

Structure	Level	Pattern	Image	Note
TS22: renal vesicle	Present		7C'

Structure	Level	Pattern	Image	Note
TS22: s-shaped body	Strong		7D'

Structure	Level	Pattern	Image	Note
TS22: early nephron	Strong		7E'

Structure	Level	Pattern	Image	Note
TS25: collecting duct	Present		7G
TS25: glomerular tuft	Absent		7G
TS25: renal proximal tubule	Absent		7G

Structure	Level	Pattern	Image	Note
TS25: epididymis	Present		8A
TS25: interstitium of the testis	Present		8A
TS25: germ cell of testis	Absent		8A

Structure	Level	Pattern	Image	Note
TS25: interstitium of the testis	Present		8B
TS25: germ cell of testis	Absent		8B
TS25: seminiferous cord	Absent		8B

Structure	Level	Pattern	Image	Note
TS25: mesenchymal stroma of ovary	Present		8C
TS25: germ cell of ovary	Absent		8C

Structure	Level	Pattern	Image	Note
TS28: ovary	Present	Regionally restricted	8D	Expression is in cells surrounding the primary follicles.
TS28: ovary follicle cell	Weak		8D