Antibody:	anti-Pax6
Assay notes:	Two antibodies were described, but the one used in this assay was not detailed. Pax6 antibody (MGI:2157358 may have been used in this assay.

Structure	Level	Pattern	Image	Note
TS15: lens pre-placode	Strong	Regionally restricted	1A	Strong expression in prospective lens ectoderm.

Structure	Level	Pattern	Image	Note
TS17: lens pit	Present	Widespread	1B	Expression in all lens pit cells.
TS17: surface ectoderm	Ambiguous		1B	Expression downregulated in surrounding surface ectoderm.

Structure	Level	Pattern	Image	Note
TS15: surface ectoderm	Strong	Regionally restricted	1C	Strong expression in prospective lens ectoderm.

Structure	Level	Pattern	Image	Note
TS17: lens pit	Present	Widespread	1D	Expression in all lens pit cells.
TS17: surface ectoderm	Moderate		1D	Expression downregulated in surrounding surface ectoderm.

Structure	Level	Pattern	Image	Note
TS19: retina	Present	Regionally restricted	3A	Expression in developing ciliary marginal zone.
TS19: lens vesicle	Present		3A
TS19: pigmented retinal epithelium	Present		3A
TS19: cornea epithelium	Present		3A	Expression in surface ectoderm that will form the cornea.

Structure	Level	Pattern	Image	Note
TS19: cornea epithelium	Present		3D	No detectable change in expression with respect to wild-type.
TS19: lens vesicle	Present		3D	No detectable change in expression with respect to wild-type.
TS19: pigmented retinal epithelium	Present		3D	No detectable change in expression with respect to wild-type.
TS19: retina	Present		3D	No detectable change in expression with respect to wild-type.

Structure	Level	Pattern	Image	Note
TS15: nose	Present	Regionally restricted	4G	Expression throughout nasal ectoderm as nasal placode begins to invaginate to form the nasal pit.

Structure	Level	Pattern	Image	Note
TS17: nasal epithelium	Present	Regionally restricted		Expression throughout nasal ectoderm as nasal placode begins to invaginate to form the nasal pit.