Probe:	probe 18.1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS13: head surface ectoderm	Strong	Regionally restricted	4A/B	Expressed in the most rostral region of the head folds.

Structure	Level	Pattern	Image	Note
TS13: trunk surface ectoderm	Strong	Regionally restricted	4C/D	Expressed in dorsal surface ectoderm in the caudal region.
TS13: trunk unsegmented mesenchyme	Strong		4C/D
TS13: intermediate mesenchyme	Strong	Regionally restricted	4C/D	Expressed in intermediate mesoderm anterior to the streak.

Structure	Level	Pattern	Image	Note
TS13: intermediate mesenchyme	Present	Regionally restricted	4E/F	Expressed in rostral trunk regions.
TS13: trunk somite	Absent		4E/F

Structure	Level	Pattern	Image	Note
TS15: myotome	Present		5A/B

Structure	Level	Pattern	Image	Note
TS17: myotome	Present		5C/D
TS17: future spinal cord	Present	Regionally restricted	5C/D	Expressed in presumptive motor neurons in the ventral horns.

Structure	Level	Pattern	Image	Note
TS22: spinal cord ventral horn	Strong		5E/F	Expressed in presumptive motor neurons.

Structure	Level	Pattern	Image	Note
TS20: trunk musculature	Present		5G/H
TS20: skeletal musculature	Present	Regionally restricted	5G/H	Expressed in most or all skeletal myoblast groups of the head.
TS20: spinal cord ventral horn	Present		5G/H	Expressed in presumptive motor neurons.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud mesenchyme	Present	Regionally restricted	6A/B	Expressed in the proximal half near the dorsal and ventral surfaces.
TS17: trunk mesenchyme	Present	Regionally restricted	6A/B	Expressed in trunk longitudinal muscle.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud apical ectodermal ridge	Present	Widespread	6C/D	Expressed along the entire anterioposterior length.
TS17: hindlimb bud mesenchyme	Present	Regionally restricted	6C/D	Expressed in the proximal half near the dorsal and ventral surfaces.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud apical ectodermal ridge	Present	Widespread	6E/F	Expressed along the entire anterioposterior length.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	6G/H	Expressed in mesenchyme beneath the dorsal and ventral surfaces of the bud in a medial zone with respect to the anterioposterior axis of the bud (dorsoventral dumbbell expression).

Structure	Level	Pattern	Image	Note
TS20: hand mesenchyme	Present	Regionally restricted	6J/L	Expressed in subsurface mesenchyme dorsal and ventral to the phalangeal condensations, which does not coexpress with Actc1.

Structure	Level	Pattern	Image	Note
TS22: foot mesenchyme	Present	Regionally restricted	6K/M	Expressed in subsurface mesenchyme distal to the phalangeal condensations, which does not coexpress with Actc1.

Structure	Level	Pattern	Image	Note
TS17: bulboventricular groove	Present	Regionally restricted	7I	Expression is most prominent in the myocardium.
TS17: bulbus cordis cardiac muscle	Present		7I	Weaker expression.
TS17: trabecular myocardium	Present		7I	Weaker expression.

Structure	Level	Pattern	Image	Note
TS17: bulboventricular groove	Present	Regionally restricted	7J	Expression is most prominent in the myocardium.
TS17: bulbus cordis cardiac muscle	Present		7J	Weaker expression.
TS17: trabecular myocardium	Present		7J	Weaker expression.
TS17: atrium cardiac muscle	Absent		7J

Structure	Level	Pattern	Image	Note
TS17: atrioventricular endocardial cushion	Absent		7K
TS17: atrium cardiac muscle	Absent		7K
TS17: bulbus cordis cardiac muscle	Present		7K	Weaker expression.
TS17: trabecular myocardium	Present		7K	Weaker expression.

Structure	Level	Pattern	Image	Note
TS17: atrium cardiac muscle	Absent		7L
TS17: pharynx	Present	Regionally restricted	7L	Expressed in the floor of the primitive pharynx, the structure giving rise to the lung buds.

Structure	Level	Pattern	Image	Note
TS20: interventricular groove	Strong		7M/N
TS20: ventricle cardiac muscle	Present	Graded	7M/N	Expression in ventricular myocardium decreases as a function of distance from the interventricular groove.

Structure	Level	Pattern	Image	Note
TS20: interventricular groove	Strong		7O/P
TS20: ventricle cardiac muscle	Present	Graded	7O/P	Expression in ventricular myocardium decreases as a function of distance from the interventricular groove.

Structure	Level	Pattern	Image	Note
TS22: interventricular groove	Weak	Regionally restricted	7Q/R	Expressed in the interventricular myocardium.

Structure	Level	Pattern	Image	Note
TS17: bronchus epithelium	Present		8A/B
TS17: pleura	Present		8A/B
TS17: gut mesenchyme	Present	Regionally restricted	8A/B	Expressed in the outer lining of the intestinal mesentery.
TS17: esophagus	Present	Regionally restricted	8A/B	Expressed in the outer linings.

Structure	Level	Pattern	Image	Note
TS20: pleura	Present		8C/D
TS20: bronchus epithelium	Absent		8C/D

Structure	Level	Pattern	Image	Note
TS17: esophagus	Present	Regionally restricted	8E/F	Expressed in the outer linings.
TS17: liver	Present	Regionally restricted	8E/F	Expressed in the outer linings.
TS17: stomach	Present	Regionally restricted	8E/F	Expressed in the outer linings.
TS17: foregut	Present	Regionally restricted	8E/F	Expressed in the outer linings of the intestinal primordia.

Structure	Level	Pattern	Image	Note
TS22: intestine mesenchyme	Present	Regionally restricted	8G/H	Expressed in the outer lining of the intestinal mesentery.
TS22: intestine epithelium	Strong	Regionally restricted	8G/H	Expressed in the luminal epithelium of the intestine.
TS22: alimentary system epithelium	Strong	Widespread	8G/H	Expressed in the lumenal epithelia along the alimentary tract.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Strong		9A/B
TS17: acoustic ganglion	Strong	Regionally restricted	9A/B	Expressed in the presumptive acoustic component.

Structure	Level	Pattern	Image	Note
TS22: semicircular canal epithelium	Present	Regionally restricted	9C/D	Expressed in the squamous epithelium. No expression was detected in the columnar epithelia.
TS22: cranial ganglion	Present	Regionally restricted	9C/D	Expressed in the acoustic ganglion.

Structure	Level	Pattern	Image	Note
TS17: neural retinal epithelium	Present	Widespread	9E/F

Structure	Level	Pattern	Image	Note
TS24: retina layer	Present	Regionally restricted	9G/H	Expressed in the differentiated ganglion layer and in the proliferating ventricular layer.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component ectoderm	Present		10A/B
TS17: 1st branchial arch maxillary component ectoderm	Present		10A/B

Structure	Level	Pattern	Image	Note
TS20: palatal shelf epithelium	Present		10C/D
TS20: tongue muscle	Present		10C/D

Structure	Level	Pattern	Image	Note
TS22: main olfactory bulb	Present	Regionally restricted	10E/F	Expressed in the postmitotic neurons which have migrated away from the ventricular zone.

Structure	Level	Pattern	Image	Note
TS22: enamel knot	Present		10G/H

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent
TS11: extraembryonic component	Absent

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Weak

Structure	Level	Pattern	Image	Note
TS15: tail unsegmented mesenchyme	Present
TS15: mesonephros	Absent
TS15: otocyst	Weak
TS15: primitive ventricle	Weak

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted		Expressed in the motor nuclei.

Structure	Level	Pattern	Image	Note
TS20: tail unsegmented mesenchyme	Present
TS20: genital swelling of genital tubercle	Absent
TS20: metanephros	Absent
TS20: limb mesenchyme	Present	Widespread
TS20: cerebral cortex	Present	Regionally restricted		Expressed in the motor nuclei.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted		Expressed in the motor nuclei.
TS22: saccule	Present
TS22: semicircular canal	Present
TS22: cochlear duct	Present	Regionally restricted		Expressed in the acoustic spiral canal.

Structure	Level	Pattern	Image	Note
TS28: endometrium epithelium	Strong