Gene Expression Data
Assay Details
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Assay
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Reference:
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J:40047
Payne J, et al., Spina bifida occulta in homozygous Patch mouse embryos. Dev Dyn. 1997 May;209(1):105-16
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5906258
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Gene symbol:
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Pdgfra
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Gene name:
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platelet derived growth factor receptor, alpha polypeptide
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Probe:
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Pdgfra probe18
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
5B:
embryonic day 9.5
(more )
Note: Section at forelimb level.
Specimen
5B:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6J
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Section at forelimb level.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk sclerotome |
Present |
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5B
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Authors state expression levels are maintained by somitic cells that become sclerotomal. Ventral to dorsal gradient of expression, consistent with sclerotomal cell migration into the region of the notochord.
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| TS15: trunk dermatome |
Weak |
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5B
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Expression levels are slightly reduced in dermatome.
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Specimen
5D:
embryonic day 11.5
(more )
Note: Section at hindlimb level.
Specimen
5D:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6J
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Section at hindlimb level.
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Specimen
5F:
embryonic day 13.0
(more )
Note: Section through lumbar trunk.
Specimen
5F:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6J
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Section through lumbar trunk.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: dermis |
Present |
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5F
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Expression in subsequent dermis, located between myotome and surface ectoderm.
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Specimen
5H:
embryonic day 14.0
(more )
Note: Section through lumbar trunk.
Specimen
5H:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6J
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Section through lumbar trunk.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: dermis |
Present |
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5H
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Expression in subsequent dermis, located between myotome and surface ectoderm.
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