Probe:	pBS/KS-Id3sp
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS19: 1st branchial arch	Present		1_panel 3
TS19: Rathke's pouch	Present		1_panel 3
TS19: somite	Present		1_panel 3
TS19: trunk mesenchyme	Present	Regionally restricted	1_panel 3	Expression is in mesenchyme surrounding the dorsal side of the developing stomach.

Structure	Level	Pattern	Image	Note
TS19: sclerotome	Present	Regionally restricted	2D	Expression is in the rostral half of the sclerotome.

Structure	Level	Pattern	Image	Note
TS24: forelimb digit	Present	Regionally restricted	2I	Expression is in the hypertrophic cartilage region and in the bony diaphysis.
TS24: radius	Present	Regionally restricted	2I	Expression is in the hypertrophic cartilage region and in the bony diaphysis.

Structure	Level	Pattern	Image	Note
TS22: cranium	Present	Regionally restricted	2N	Expression is in the cranio-facial skeleton.
TS22: jaw skeleton	Present	Regionally restricted	2N	Expression is in the cranio-facial skeleton.
TS22: palatal shelf	Present	Regionally restricted	2N	Expression is in ossification centers.
TS22: Meckel's cartilage	Present		2N
TS22: hyoid cartilage primordium	Present		2N
TS22: nasal septum	Present		2N
TS22: otic capsule	Present		2N
TS22: sphenoid bone	Present		2N

Structure	Level	Pattern	Image	Note
TS20: hindlimb interdigital region mesenchyme	Present		2S
TS20: hindlimb pre-cartilage condensation	Present	Regionally restricted	2S	Expression is in the blastemal condenstaions where chondrification has started.

Structure	Level	Pattern	Image	Note
TS24: dental papilla	Present	Regionally restricted	3_panel 3	Expression is in the dental papilla mesenchyme and is absent in the ondontoblasts.
TS24: enamel organ	Present	Regionally restricted	3_panel 3	Expression is in the ameloblast layer.
TS24: stellate reticulum	Present		3_panel 3

Structure	Level	Pattern	Image	Note
TS19: stomach	Present	Regionally restricted	5C	Expression is in the stomach endoderm in the dorsal part.
TS19: stomach mesenchyme	Present	Regionally restricted	5C	Expression is in mesenchyme surrounding the endoderm in the dorsal part.

Structure	Level	Pattern	Image	Note
TS22: intestine muscularis	Present		5H
TS22: intestine mesenchyme	Present	Regionally restricted	5H	Expression is in mesenchymal cells adjacent to the epithelium.

Structure	Level	Pattern	Image	Note
TS24: lung mesenchyme	Present	Regionally restricted	6B/E	Expression is in the mesenchymal cells surrounding the main bronchi and the smaller bronchi/bronchioles.

Structure	Level	Pattern	Image	Note
TS22: metanephros	Present	Regionally restricted	6H/K	Expression is in the condensed metanephric mesenchyme.
TS22: collecting duct	Present		6H/K
TS22: glomerular capillary system of maturing glomerular tuft	Present		6H/K

Structure	Level	Pattern	Image	Note
TS19: endocardial cushion	Strong		7_panel 3
TS19: atrioventricular endocardial cushion	Moderate		7_panel 3
TS19: outflow tract	Moderate		7_panel 3

Structure	Level	Pattern	Image	Note
TS22: hyaloid vascular plexus	Present	Regionally restricted	8C/G

Structure	Level	Pattern	Image	Note
TS22: salivary gland mesenchyme	Present	Regionally restricted	9C/G	Expression is in the mesenchymal cells surrounding the epithelium in the developing salivary gland.

Structure	Level	Pattern	Image	Note
TS24: thyroid gland	Present		9I/J
TS24: parathyroid gland	Absent		9I/J

Structure	Level	Pattern	Image	Note
TS13: future brain neural fold	Present	Regionally restricted		Expression is in the ridges of the unclosed neural folds.
TS13: head mesenchyme	Present	Regionally restricted		Expression is in the cranial mesenchyme underlying the unclosed neural folds.

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present
TS15: limb	Present
TS15: trunk mesenchyme	Very strong	Regionally restricted		Expression is in the mesechyme surrounding the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS17: limb	Present
TS17: stomach mesenchyme	Present	Regionally restricted		Expression is in mesenchyme surrounding the endoderm in the dorsal part.
TS17: stomach	Present	Regionally restricted		Expression is in the stomach endoderm in the dorsal part.
TS17: hindgut mesenchyme	Present	Regionally restricted		Expression is in the mesenchyme surrounding the gut epithelium.
TS17: midgut mesenchyme	Present	Regionally restricted		Expression is in the mesenchyme surrounding the gut epithelium.
TS17: trunk mesenchyme	Very strong	Regionally restricted		Expression is in the mesechyme surrounding the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS19: brain blood vessel	Present	Regionally restricted		Expression is in the blood vessels of the meninges.
TS19: stomach	Present	Regionally restricted		Expression is in the stomach endoderm in the dorsal part.
TS19: stomach mesenchyme	Present	Regionally restricted		Expression is in mesenchyme surrounding the endoderm in the dorsal part. `
TS19: nasal process	Present
TS19: stomach epithelium	Present

Structure	Level	Pattern	Image	Note
TS20: rib pre-cartilage condensation	Present	Regionally restricted		Expression is in the perichondrium.
TS20: jaw	Present	Regionally restricted		Expression is in a patch of toothbud epithelium and is weak in the condensed mesenchymal cells.
TS20: vibrissa mesenchyme	Present	Regionally restricted		Expression is in the condensing mesenchyme underneath the epidermal placodes.
TS20: metanephros	Present	Regionally restricted		Expression is in the condensed metanephric mesenchyme and renal pelvis, absent in the ureteric bud.
TS20: pancreas	Present

Structure	Level	Pattern	Image	Note
TS22: inner enamel epithelium	Very strong
TS22: outer enamel epithelium	Very strong
TS22: stellate reticulum	Very strong
TS22: tooth mesenchyme	Very strong	Regionally restricted		Expression is in the condensed mesenchyme.
TS22: cap stage tooth	Very strong	Regionally restricted

Structure	Level	Pattern	Image	Note
TS22: stomach mesenchyme	Present
TS22: stomach glandular region	Present	Regionally restricted		Expression is in the glandular mucosa.
TS22: stomach epithelium	Weak

Structure	Level	Pattern	Image	Note
TS24: brain blood vessel	Present
TS24: collecting duct	Present
TS24: glomerular capillary system of maturing glomerular tuft	Present
TS24: heart valve	Present
TS24: mesenchyme of metanephros	Present
TS24: renal pelvis	Present
TS24: vibrissa dermal papilla	Weak
TS24: vibrissa outer root sheath	Weak
TS24: vibrissa dermal component	Weak	Regionally restricted		Expression is in the dermal sheath region.
TS24: intestine	Trace