Probe:	mRGM A probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS22: olfactory cortex	Present	Regionally restricted	2A	Expression in olfactory tubercle.
TS22: piriform cortex	Present		2A
TS22: eye	Absent		2A

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Strong	Regionally restricted	2B	Strong expression in cortical neuroepithelium.
TS22: hippocampus	Present	Regionally restricted	2B	Increasing ventro-dorsal gradient peaking in hippocampal ventricular layer.
TS22: amygdala	Present		2B
TS22: corpus striatum	Present		2B
TS22: cerebellum	Present		2B
TS22: cochlear duct epithelium	Present		2B
TS22: lung	Present	Regionally restricted	2B	Expression in bronchial epithelium in bronchi of medium and small caliber.
TS22: limb cartilage condensation	Present		2B

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	2C	In the cortex, expression in the neuroepithelium.
TS22: main olfactory bulb mitral cell layer	Present		2C	Expression in a restricted area of mantle corresponding to presumptive mitral and ventricular layers.
TS22: main olfactory bulb ventricular layer	Present		2C	Expression in a restricted area of mantle corresponding to presumptive mitral and ventricular layers.
TS22: telencephalon septum	Present		2C
TS22: dorsal thalamus	Present	Regionally restricted	2C	Authors report patterned expression in dorsal and ventral thalamus, as well as hypothalamus.
TS22: hypothalamus	Present	Regionally restricted	2C	Authors report patterned expression in dorsal and ventral thalamus, as well as hypothalamus.
TS22: brainstem	Present	Scattered	2C
TS22: spinal cord	Present	Scattered	2C

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	2D	In the cortex, expression in the neuroepithelium.
TS22: prethalamus	Present	Regionally restricted	2D	Authors report patterned expression in dorsal and ventral thalamus, as well as hypothalamus.
TS22: diencephalon	Present	Regionally restricted	2D	Regional expression in neuroepithelium lining the third ventricle.
TS22: pretectum	Present		2D
TS22: midbrain tegmentum	Present	Regionally restricted	2D	Expression in tectal and tegmental mantle of midbrain.
TS22: tectum	Present	Regionally restricted	2D	Expression in tectal and tegmental mantle of midbrain.
TS22: hindbrain mantle layer	Present	Regionally restricted	2D	In hindbrain mantle, most intense expression in inferior olive.
TS22: brainstem	Present	Scattered	2D
TS22: hindbrain	Weak	Regionally restricted	2D	Intense homogeneous expression in neuroepithelial cells lining the aqueduct, fourth ventricle and central canal of spinal cord.
TS22: midbrain	Weak	Regionally restricted	2D	Intense homogeneous expression in neuroepithelial cells lining the aqueduct, fourth ventricle and central canal of spinal cord.
TS22: spinal cord	Weak	Regionally restricted	2D	Intense homogeneous expression in neuroepithelial cells lining the aqueduct, fourth ventricle and central canal of spinal cord.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	2E	In the cortex, expression in the neuroepithelium.
TS22: dorsal thalamus	Present	Regionally restricted	2E	Authors report patterned expression in dorsal and ventral thalamus, as well as hypothalamus.
TS22: hypothalamus	Present	Regionally restricted	2E	Authors report patterned expression in dorsal and ventral thalamus, as well as hypothalamus.
TS22: prethalamus	Present	Regionally restricted	2E	Authors report patterned expression in dorsal and ventral thalamus, as well as hypothalamus.
TS22: mammillary area	Present		2E
TS22: pretectum	Present		2E
TS22: midbrain tegmentum	Present	Regionally restricted	2E	Expression in tectal and tegmental mantle of midbrain.
TS22: tectum	Present	Regionally restricted	2E	Expression in tectal and tegmental mantle of midbrain.
TS22: midbrain	Strong	Regionally restricted	2E	Intense homogeneous expression in neuroepithelial cells lining the aqueduct, fourth ventricle and central canal of spinal cord.
TS22: spinal cord	Strong	Regionally restricted	2E	Intense homogeneous expression in neuroepithelial cells lining the aqueduct, fourth ventricle and central canal of spinal cord.
TS22: cerebellum	Present		2E
TS22: hindbrain	Present	Regionally restricted	2E	Prominent expression in inferior olive.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct epithelium	Present		2F

Structure	Level	Pattern	Image	Note
TS22: telencephalon septum	Present		2G
TS22: hypothalamus	Present	Regionally restricted	2G	Expression in anterior hypothalamic area.
TS22: dorsal thalamus	Present		2G
TS22: mammillary area	Present		2G
TS22: preoptic area	Present		2G

Structure	Level	Pattern	Image	Note
TS22: midbrain ventricular layer	Strong	Regionally restricted	2H
TS22: midbrain tegmentum	Weak	Regionally restricted	2H	Weak expression in tectal and tegmental mantle.
TS22: tectum	Weak	Regionally restricted	2H	Weak expression in tectal and tegmental mantle.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present		2I
TS22: hindbrain ventricular layer	Present		2I

Structure	Level	Pattern	Image	Note
TS25: brain mantle layer	Present	Widespread
TS25: ventricular layer	Absent

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Strong		3C
TS28: cortical layer II	Strong		3C
TS28: cortical layer III	Strong		3C
TS28: cortical layer V	Strong		3C
TS28: cortical layer VI	Present		3C
TS28: basal ganglia	Present	Scattered	3C
TS28: piriform cortex	Strong		3C
TS28: caudate-putamen	Present	Scattered	3C
TS28: globus pallidus	Present	Scattered	3C
TS28: basal forebrain	Moderate	Widespread	3C
TS28: anterior dorsal thalamic nucleus	Present		3C	Regionally but differentially in hippocampal and thalamic subregions.
TS28: medial dorsal thalamic nucleus	Present		3C	Regionally but differentially in hippocampal and thalamic subregions.
TS28: nucleus reuniens	Present		3C	Regionally but differentially in hippocampal and thalamic subregions.
TS28: reticular thalamic nucleus	Present		3C	Regionally but differentially in hippocampal and thalamic subregions.
TS28: hippocampus CA1	Strong		3C	Expression most intense in CA1.
TS28: hippocampus CA3	Present		3C
TS28: hippocampus	Present	Scattered	3C	Expression in scattered hilar cells.
TS28: dentate gyrus	Absent		3C
TS28: hippocampus stratum oriens	Absent		3C

Structure	Level	Pattern	Image	Note
TS28: lateral habenular nucleus	Weak		3D
TS28: medial dorsal thalamic nucleus	Weak		3D
TS28: medial habenular nucleus	Weak		3D