Gene Expression Data
Assay Details
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Assay
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Reference:
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J:89166
Oosterveen T, et al., Expression of retinaldehyde dehydrogenase II and sequential activation of 5' Hoxb genes in the mouse caudal hindbrain. Gene Expr Patterns. 2004 May;4(3):243-7
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5905594
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Gene symbol:
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Hoxb6
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Gene name:
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homeobox B6
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Probe:
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Hoxb6 probe13
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1D:
embryonic day 9.5
(more  )
Specimen
1D:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube |
Present |
Regionally restricted |
1D
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Anterior boundary of expression domain at level of posterior part of somite 5, just anterior to the somite 5/6 boundary, which is the position of the first dorsal root ganglion.
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Specimen
1E:
embryonic day 10.5
(more )
Specimen
1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Present |
Regionally restricted |
1E
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Anterior boundary of expression caudal to otic vesicle.
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Specimen
1F:
embryonic day 11.5
(more )
Specimen
1F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: neural tube |
Present |
Regionally restricted |
1F
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Authors state the that the distance between otic vesicle and the most anterior expressing cells is reduced 1.5-fold between E9.5 and E11.5.
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Specimen
2D:
embryonic day 9.5
(more )
Note: Double labeled: blue - Hoxb6; orange - Raldh2 (Aldh1a2 assay MGI:5905599).
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: blue - Hoxb6; orange - Raldh2 (Aldh1a2 assay MGI:5905599).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube |
Present |
Regionally restricted |
2D
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Anterior boundary of expression in neurectoderm near posterior part of somite 5, just anterior to the somite 5/6 boundary, which is the position of the first dorsal root ganglion.
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Specimen
2I:
embryonic day 10.5
(more )
Note: Double labeled: blue - Hoxb6; orange - Raldh2 (Aldh1a2 assay MGI:5905599).
Specimen
2I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: blue - Hoxb6; orange - Raldh2 (Aldh1a2 assay MGI:5905599).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Present |
Regionally restricted |
2I
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Rostral boundary of expression located more rostrally than anterior expression limit of Raldh2 (Aldh1a2).
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| TS17: spinal ganglion |
Present |
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2I
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Specimen
3C:
embryonic day 11.5
(more )
Specimen
3C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: neural tube |
Present |
Regionally restricted |
3C
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Anteiror boundary of expression domain located more rostrally than a day earlier, and more anterior than the cervical Raldh2 (Aldh1a2) domain.
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Specimen
3D:
embryonic day 11.5
(more )
Note: Double labeled: darker blue - Hoxb6; lighter blue - Raldh2 (Aldh1a2 assay MGI:5905597).
Specimen
3D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: darker blue - Hoxb6; lighter blue - Raldh2 (Aldh1a2 assay MGI:5905597).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: neural tube |
Present |
Regionally restricted |
3D
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Authors report expression boundary is much more rostral compared with earlier stages.
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Analysis Tools