Gene Expression Data
Assay Details
Assay
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Reference:
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J:89166
Oosterveen T, et al., Expression of retinaldehyde dehydrogenase II and sequential activation of 5' Hoxb genes in the mouse caudal hindbrain. Gene Expr Patterns. 2004 May;4(3):243-7
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5905593
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Gene symbol:
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Hoxb5
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Gene name:
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homeobox B5
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Probe:
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Hoxb5 probe7
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A:
embryonic day 9.5
(more )
Specimen
1A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS15: neural tube |
Present |
Regionally restricted |
1A
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Anterior expression boundary caudal to otic vesicle. Distance between the rostral most expressing neural tissue and the otic vesicle is indicated in the image.
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Specimen
1B:
embryonic day 10.5
(more )
Specimen
1B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
|
Sex:
|
Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: neural tube |
Present |
Regionally restricted |
1B
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Authors report the rostral boundary of expression domain is more diffuse at E10.5 than at E11.5.
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Specimen
1C:
embryonic day 11.5
(more )
Specimen
1C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: neural tube |
Present |
Regionally restricted |
1C
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Authors state the that the distance between otic vesicle and the most anterior expressing cells is reduced 2-fold between E9.5 and E11.5.
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Specimen
2A:
embryonic day 9.5
(more )
Specimen
2A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: neural tube |
Present |
Regionally restricted |
2A
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Expression up to level of somite 4 in the neural tube.
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Specimen
2C:
embryonic day 9.5
(more )
Note: Double labeled: blue - Hoxb5; orange - Raldh2 (Aldh1a2 assay MGI:5905599).
Specimen
2C:
(close )
Genetic Background:
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Not Specified
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
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Double labeled: blue - Hoxb5; orange - Raldh2 (Aldh1a2 assay MGI:5905599).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: neural tube |
Present |
Regionally restricted |
2C
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Expression up to level of somite 4 in the neural tube.
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Specimen
2F:
embryonic day 10.5
(more )
Specimen
2F:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: neural tube |
Present |
Regionally restricted |
2F
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Rostral boundary of expression located more rostrally than anterior expression limit of Raldh2 (Aldh1a2).
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Specimen
2H:
embryonic day 10.5
(more )
Note: Double labeled: blue - Hoxb5; orange - Raldh2 (Aldh1a2 assay MGI:5905599).
Specimen
2H:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: blue - Hoxb5; orange - Raldh2 (Aldh1a2 assay MGI:5905599).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: neural tube |
Present |
Regionally restricted |
2H
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Rostral boundary of expression located more rostrally than anterior expression limit of Raldh2 (Aldh1a2).
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TS17: spinal ganglion |
Present |
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2H
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