Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Regionally restricted	2a	Expression at the midline, along the entire longitudinal axis of the embryo.
TS15: paraxial mesenchyme	Weak	Regionally restricted	2a	Weak expression in intersomitic mesenchyme.
TS15: head	Present	Regionally restricted	2a	Expression as two broad stripes at hindbrain level corresponding to rhombomeres 2 and 3.
TS15: 1st branchial arch	Present		2a
TS15: 2nd branchial arch	Present		2a
TS15: 3rd branchial arch	Present		2a
TS15: heart	Present		2a
TS15: central nervous system	Absent		2a	No apparent expression in the neural tube.

Structure	Level	Pattern	Image	Note
TS17: paraxial mesenchyme	Strong	Regionally restricted	2b	Strong expression in intersomitic mesenchyme - evolved into a distinct striped patten.

Structure	Level	Pattern	Image	Note
TS17: brain blood vessel	Present		2c

Structure	Level	Pattern	Image	Note
TS17: tail blood vessel	Present	Regionally restricted	2d	Expression in intersomitic blood vessels of tail.

Structure	Level	Pattern	Image	Note
TS17: blood vessel	Present		2e
TS17: neural tube floor plate	Present		2e
TS17: neural tube roof plate	Present		2e
TS17: vascular plexus	Present	Regionally restricted	2e	Expression in perineural vascular plexus.
TS17: peripheral nerve trunk	Present	Regionally restricted	2e	Expression in cervical nerve trunk.
TS17: notochord	Weak		2e

Structure	Level	Pattern	Image	Note
TS17: blood vessel	Present		2f	Expression in vasculature.
TS17: neural tube floor plate	Present		2f
TS17: neural tube roof plate	Present		2f
TS17: peripheral nerve trunk	Present	Regionally restricted	2f	Expression in cervical nerve trunk.

Structure	Level	Pattern	Image	Note
TS17: blood vessel	Present		2g	Expression in vasculature.
TS17: peripheral nerve trunk	Present	Regionally restricted	2g	Expression in cervical nerve trunk.
TS17: paraxial mesenchyme	Present	Regionally restricted	2g	Expression in mesenchyme surrounding somites.
TS17: somite	Absent		2g

Structure	Level	Pattern	Image	Note
TS17: cardinal vein	Strong	Spotted	2h
TS17: dorsal aorta	Weak		2h	Somewhat lesser expression in dorsal aorta.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present	Widespread	3a	Expression observed throughout embryo.
TS22: brain blood vessel	Strong	Regionally restricted	3a	Particularly strong expression in vessels of central nervous system.

Structure	Level	Pattern	Image	Note
TS22: brain blood vessel	Present	Regionally restricted	3b	Expression in brain capillaries.

Structure	Level	Pattern	Image	Note
TS22: tongue muscle	Present	Regionally restricted	3c	Vascular expression in skeletal muscle.

Structure	Level	Pattern	Image	Note
TS22: cardiac muscle tissue	Strong		3d	Strong expression in myocardium and endocardium.
TS22: endocardial lining	Strong		3d	Strong expression in myocardium and endocardium.

Structure	Level	Pattern	Image	Note
TS22: blood vessel	Present	Regionally restricted	3e	Expression in large vessels.

Structure	Level	Pattern	Image	Note
TS22: blood vessel	Present		3f
TS22: lung epithelium	Absent		3f

Structure	Level	Pattern	Image	Note
TS22: foregut vascular element	Present	Regionally restricted	3g	Vascular expression in foregut submucosa.
TS22: gut epithelium	Present	Spotted	3g

Structure	Level	Pattern	Image	Note
TS22: skin	Present	Regionally restricted	3h	Expression in vasculature of dermis and in solitary cells.

Structure	Level	Pattern	Image	Note
TS22: liver	Present	Single cells	3i	Expression in solitary cells in liver.

Structure	Level	Pattern	Image	Note
TS22: thoracic segment of trunk	Present	Regionally restricted	3j	Intercostal vascular expression.

Structure	Level	Pattern	Image	Note
TS22: maturing glomerular tuft	Present		3k	Glomerular, mesenchymal and vascular expression in kidney.
TS22: mesenchyme of metanephros	Present		3k	Glomerular, mesenchymal and vascular expression in kidney.
TS22: renal small blood vessel	Present		3k	Glomerular, mesenchymal and vascular expression in kidney.

Structure	Level	Pattern	Image	Note
TS28: spleen	Present	Regionally restricted	4a	Vascular expression in spleen. Expression in a large number of small caliber vessels. Expression also in solitary cells.

Structure	Level	Pattern	Image	Note
TS28: brain blood vessel	Present	Regionally restricted	4b	Expression in several large vessels (arteries/veins) and arterioles/venules.No expression in brain capillaries.

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present	Single cells	4c	Expression in solitary cells localized preferentially in CD113 (Nectin3)-positive areas in walls of lateral ventricles. Some cells outside of these areas.

Structure	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	Present	Spotted	4d
TS28: heart blood vessel	Present		4d

Structure	Level	Pattern	Image	Note
TS28: lung parenchyma	Present	Regionally restricted	4e	Expression in lung mesenchyme and macrophages.

Structure	Level	Pattern	Image	Note
TS28: liver vascular element	Present	Regionally restricted	4f	Expression only in large blood vessels of liver.

Structure	Level	Pattern	Image	Note
TS28: white fat	Present	Regionally restricted	4g	Expression in a few large vessels in white adipose tissue.

Structure	Level	Pattern	Image	Note
TS28: brown fat	Present	Regionally restricted	4h	Expression in brown adipose vasculature, likely representing capillary blood vessels.

Structure	Level	Pattern	Image	Note
TS28: pancreas	Present	Regionally restricted	4i	Expression in a few large blood vessels in the pancreas.

Structure	Level	Pattern	Image	Note
TS28: glomerular tuft	Present		4j

Structure	Level	Pattern	Image	Note
TS28: glomerular tuft	Present		4k
TS28: renal small blood vessel	Present		4k

Structure	Level	Pattern	Image	Note
TS28: skeletal muscle	Present	Regionally restricted	4l	Expression in a few of small caliber blood vessels in skeletal muscle.

Structure	Level	Pattern	Image	Note
TS28: retina blood vessel	Strong	Regionally restricted	5c	Strong expression primarily in retinal veins and expanding capillary plexus. Weak spotty arterial expression.

Structure	Level	Pattern	Image	Note
TS28: retina blood vessel	Strong	Regionally restricted	5f	Strong arterial expression - large arteries and their principal branches. Weak capillary expression.

Structure	Level	Pattern	Image	Note
TS28: retina blood vessel	Present	Regionally restricted	5h	Arterial expression - large arteries and their principal branches. No expression in veins or capillaries.

Structure	Level	Pattern	Image	Note
TS28: middle cerebral artery	Present		5k
TS28: brain blood vessel	Present	Regionally restricted	5k	Expression in other arteries. No expression in veins.

Structure	Level	Pattern	Image	Note
TS28: middle cerebral artery	Present		5n
TS28: brain blood vessel	Present	Regionally restricted	5n	Expression in other arteries. No expression in veins.

Structure	Level	Pattern	Image	Note
TS28: arcuate artery	Present		5o
TS28: glomerular tuft	Present		5o
TS28: ureter	Present		5o
TS28: arcuate vein	Absent		5o

Structure	Level	Pattern	Image	Note
TS22: central nervous system vascular element	Present	Widespread	6a	Expression in essentially all vessels.

Structure	Level	Pattern	Image	Note
TS22: central nervous system vascular element	Present	Regionally restricted	6b	Authors report expression overlaps endothelial markers.

Structure	Level	Pattern	Image	Note
TS22: central nervous system vascular element	Present	Regionally restricted	6d	No overlap with mural cell marker NG2 (Cspg4).

Structure	Level	Pattern	Image	Note
TS22: central nervous system vascular element	Present	Regionally restricted	6e	No overlap with mural cell marker NG2 (Cspg4).

Structure	Level	Pattern	Image	Note
TS22: central nervous system vascular element	Present	Regionally restricted	6f	No overlap with mural cell marker NG2 (Cspg4).

Structure	Level	Pattern	Image	Note
TS28: central nervous system vascular element	Present	Widespread	6g	Expression in most, but not all, blood vessels.

Structure	Level	Pattern	Image	Note
TS28: central nervous system vascular element	Present	Widespread	6h	Expression in most, but not all, blood vessels.

Structure	Level	Pattern	Image	Note
TS28: central nervous system vascular element	Present	Widespread	6i	Expression in most, but not all, blood vessels.

Structure	Level	Pattern	Image	Note
TS28: central nervous system vascular element	Present	Widespread	6j	Expression in most, but not all, blood vessels.

Structure	Level	Pattern	Image	Note
TS28: central nervous system vascular element	Present	Regionally restricted	6m	Expression in a subset of vascular smooth muscle cells.

Structure	Level	Pattern	Image	Note
TS28: brain blood vessel	Present	Regionally restricted	7a	Expression in only a few vessels, primarily in arterial vascular smooth muscle cells.

Structure	Level	Pattern	Image	Note
TS28: brain blood vessel	Present	Regionally restricted	7b	Authors report expression in cells enclosing the endothelium.

Structure	Level	Pattern	Image	Note
TS28: retina blood vessel	Strong	Regionally restricted	7e	Strong expression in pericytes.

Structure	Level	Pattern	Image	Note
TS28: brain blood vessel	Present	Regionally restricted	7f	Expression in vascular smooth muscle.

Structure	Level	Pattern	Image	Note
TS28: renal small blood vessel	Present	Regionally restricted	7g	Expression in vascular smooth muscle cells of large vessels.

Structure	Level	Pattern	Image	Note
TS28: lung	Present	Regionally restricted	8b	Expression in approximatesly 25% of CD45 (Ptprc)-positive hematopoietic cells.

Structure	Level	Pattern	Image	Note
TS28: retina blood vessel	Present	Regionally restricted	8c	Expression in vascular structures (endothelial cells) as well as solitary cells with extensive cellular protrusions (microglia).

Structure	Level	Pattern	Image	Note
TS22: liver	Present	Spotted	8d	Spotty expression dispersed thoughout liver tissue, not associated with vessel structures.
TS22: liver vasculature	Present	Regionally restricted	8d	Expression only occasionally associated with CD31 (Pecam1)-positive blood vessels.