Probe:	Spry2 probe5
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS16: left lung rudiment epithelium	Present	Regionally restricted	1A	Expression in epithelium of primary bronchial buds. Expression domain larger in right bronchus than in left.
TS16: right lung rudiment epithelium	Present	Regionally restricted	1A	Expression in epithelium of primary bronchial buds. Expression domain larger in right bronchus than in left.
TS16: foregut mesenchyme	Weak	Regionally restricted	1A	Weaker expression in mesenchyme around trachea and stomach.
TS16: trachea mesenchyme	Weak	Regionally restricted	1A	Weaker expression in mesenchyme around trachea and stomach.

Structure	Level	Pattern	Image	Note
TS17: lung rudiment mesenchyme	Weak	Regionally restricted		Low level of expression in mesenchyme of distal lung buds.

Structure	Level	Pattern	Image	Note
TS18: right lung rudiment epithelium	Present	Regionally restricted	1B	Expression restricted to distal epithelium during secondary branching of lobar (right lung) bronchi.

Structure	Level	Pattern	Image	Note
TS19: left lung rudiment epithelium	Present	Regionally restricted	1C	Expression restricted to distal epithelium during secondary branching of primary segmental bronchi (left lung).

Structure	Level	Pattern	Image	Note
TS20: lobar bronchus epithelium	Present	Regionally restricted	1D

Structure	Level	Pattern	Image	Note
TS18: left lung rudiment epithelium	Present		1E

Structure	Level	Pattern	Image	Note
TS19: left lung rudiment epithelium	Present		1F	Authors state expression at bud tip increases during bud outgrowth.

Structure	Level	Pattern	Image	Note
TS19: left lung rudiment epithelium	Present		1G	Weak expression at site of emergence of a nascent lateral bronchial bud.

Structure	Level	Pattern	Image	Note
TS20: lung epithelium	Present	Regionally restricted	1H	Expression restricted to distal epithelium, increasing to a peak just before bud the tip splits.

Structure	Level	Pattern	Image	Note
TS20: lung epithelium	Present	Regionally restricted	1I

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Strong	Regionally restricted	1J	High levels of expression in distal epithelium at periphery of each lobe.

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Present	Regionally restricted	1L	Low level of expression detected throughout epithelium of each lobe, including branch tips deep within the lung.Low level of expression detected throughout epithelium of each lobe, including branch tips deep within the lung.

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Present	Regionally restricted	3D (N)	Expression in peripheral epithelium.

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Present	Regionally restricted	3E	Expression in peripheral epithelium.