Probe:	probe A
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Present		6a/a'
TS15: diencephalon	Present	Regionally restricted	6a/a'	Expressed in two nonoverlapping regions. Located anteriorly in the hypothalamus at the level of the optic stalk, and more posteriorly in an extended region in the posterior hypothalamus.

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Present		6b/b'
TS15: diencephalon	Present	Regionally restricted	6b/b'	Expressed in two nonoverlapping regions.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present		6c/c'
TS17: hindbrain	Present	Regionally restricted	6c/c'	Expression boundary is very close, or coincident, with the midbrain posterior border.
TS17: diencephalon	Present	Regionally restricted	6c/c'	Expressed in two nonoverlapping regions. Located anteriorly in the hypothalamus at the level of the optic stalk, and more posteriorly in an extended region in the posterior hypothalamus.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present		6d/d'
TS17: future spinal cord	Present		6d/d'

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	6e/e'	Expressed in two nonoverlapping regions. Located anteriorly in the hypothalamus at the level of the optic stalk, and more posteriorly in an extended region in the posterior hypothalamus.

Structure	Level	Pattern	Image	Note
TS17: hindbrain lateral wall	Present	Regionally restricted	6f/f'	Expressed in a mediolateral group of cells in the basal plate.
TS17: diencephalon	Present	Regionally restricted	6f/f'	Expressed in two nonoverlapping regions. Located anteriorly in the hypothalamus at the level of the optic stalk, and more posteriorly in an extended region in the posterior hypothalamus.

Structure	Level	Pattern	Image	Note
TS17: hindbrain lateral wall	Present	Regionally restricted	6g/g'	Expressed in a mediolateral group of cells in the basal plate.
TS17: diencephalon	Present	Regionally restricted	6g/g'	Expressed in two nonoverlapping regions. Located anteriorly in the hypothalamus at the level of the optic stalk, and more posteriorly in an extended region in the posterior hypothalamus.

Structure	Level	Pattern	Image	Note
TS16: future spinal cord	Present	Regionally restricted	7a/a'	Expressed in a medioventral region close to the sulcus limitans, excluding the internal germinative layer.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	7b/b'	Expressed in a narrow band of cells in the alar plate.

Structure	Level	Pattern	Image	Note
TS20: alar columns	Strong	Regionally restricted	7c/c'	Expression is concentrated not only in the medial regions, but appears also in more dorsal and ventral regions. Dorsally, these cells run laterally to the germinal layer.
TS20: basal columns	Strong	Regionally restricted	7c/c'	Expression is concentrated not only in the medial regions, but appears also in more dorsal and ventral regions. Dorsally, these cells run laterally to the germinal layer.

Structure	Level	Pattern	Image	Note
TS22: spinal cord lateral wall	Present	Regionally restricted	7d/d'	Expressed in the intermedial region of the lateral horn.

Structure	Level	Pattern	Image	Note
TS25: spinal cord lateral wall	Present	Regionally restricted	7e/e'	Expressed in the intermedial region of the lateral horn (visceral sensory area).

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	8a/a'	Expressed in three nonoverlapping regions.
TS20: hypothalamus	Present	Regionally restricted	8a/a'	Strongest expression in the anterior hypothalamus around the optic staik and includes the posterior preoptic area and the supraoptic paraventricular region. Posteriorly, expressed in the retrochiasmatic region, in the ventral tuberal region, and dorsally in a narrow stripe running among mammillary, retrochiasmatic, and posterior entopenduncolar areas.
TS20: preoptic area	Present	Regionally restricted	8a/a'	Expressed in the posterior preoptic area.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	8b/b'	Expressed in three nonoverlapping regions.
TS20: hypothalamus	Present	Regionally restricted	8b/b'	Expressed in the retrochiasmatic region, in the ventral tuberal region, and dorsally in a narrow stripe running among mammillary, retrochiasmatic, and posterior entopenduncolar areas.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	8c/c'	Expressedd in the inferior part of the massa cellularis reuniens, which is the matrix of the globus pallidus and the stria terminalis.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present		8d/d'	Most strongly.
TS20: optic stalk	Present		8d/d'	Most strongly.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	8e/e'	Expressed in the postoptic region and attributed to the retrochiasmatic area.

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present		8f/f'
TS20: midbrain-hindbrain junction	Present	Regionally restricted	8f/f'	Expressed in the rhombic isthmus at the ventral part.
TS20: medulla oblongata basal plate	Present		8f/f'
TS20: medulla oblongata lateral wall	Present	Regionally restricted	8f/f'	Expressed only in the basal plate and not in the more medial zone.

Structure	Level	Pattern	Image	Note
TS20: pons	Present	Regionally restricted	8g/g'	Expressed only in the basal plate and not in the more medial zone.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	8h/h'	Expressed in the retrochiasmatic region, in the ventral tuberal region, and dorsally in a narrow stripe running among mammillary, retrochiasmatic, and posterior entopenduncolar areas.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	8i/i'	Sharply expressed in the supraoptic paraventricular and postoptic retrochiasmatic regions.

Structure	Level	Pattern	Image	Note
TS25: hypothalamus	Present	Regionally restricted	9a/a'	Expressed in the well-developed preoptic and postoptic areas.
TS25: preoptic area	Present		9a/a'

Structure	Level	Pattern	Image	Note
TS25: preoptic area	Present	Regionally restricted	9b/b'	Expressed in the presumptive preoptic lateral and preoptic periventricular areas.

Structure	Level	Pattern	Image	Note
TS25: hypothalamus	Present	Regionally restricted	9c/c'	Expressed in the presumptive paraventricular supraoptic and arcuatus nuclei.
TS25: telencephalon	Present	Regionally restricted	9c/c'	Expressed in the presumptive stria terminalis.
TS25: optic tract	Present		9c/c'

Structure	Level	Pattern	Image	Note
TS25: hypothalamus	Present	Regionally restricted	9d/d'	Expressed in the presumptive paraventricular supraoptic and arcuatus nuclei.
TS25: telencephalon	Present	Regionally restricted	9d/d'	Expressed in the amygdaloid complex, and a sharp ribbon, located ventrally to the zona incerta.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	10d	Expressed in the massa cellularis reuniens pars inferior and probably the supraoptic-paraventricular area.
TS20: telencephalon	Present	Regionally restricted	10d	Expressed in the globus pallidus anlage, included in a large Emx2-expressing region.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	10h/h'	Otx7 and Otp are expressed in close but not overlapping domains. The thin stripe of cells expressing Otx7 defines ventrally the Otp expression. No expression in the periventricular germinal layers.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	10l/l'	Expressed in the retrochiasmatic region.

Structure	Level	Pattern	Image	Note
TS20: metencephalon	Present		11b/b'	Otx2 expression limit is coincident but not is overlapping with the Otp anterior limit.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present		11d/d'	Otp and D1x1 show adjacent expression domains along the dorsoventral axis.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	11f/f'	Expressed anteriorly in a sharp stripe corresponding to the preoptic posterior area and is expressed posteriorly in the retrochiasmatic area. The anterior Otp stripe is flanked by two Dlx1 domains; the posterior Dlx1 stripe is flanked by two Otp domains.

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present			Almost identically expressed as in E12.5.
TS22: spinal cord	Present			Almost identically expressed as in E12.5.

Structure	Level	Pattern	Image	Note
TS25: hindbrain	Present	Regionally restricted		Lower expression in the basal hindbrain derivatives.