Probe:	MC-1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme derived from neural crest	Present		1A/B	Expression first detected in cells between surface ectoderm and brain neuroepithelium in head region - head crest.

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme derived from neural crest	Present	Regionally restricted	1C	Expression in region immediately caudal to otic vesicle - vagal crest.

Structure	Level	Pattern	Image	Note
TS15: trunk mesenchyme derived from neural crest	Absent		1D

Structure	Level	Pattern	Image	Note
TS15: tail mesenchyme derived from neural crest	Absent		1E

Structure	Level	Pattern	Image	Note
TS15: trunk mesenchyme derived from neural crest	Present			In trunk region, expression not seen until 27-28 somite stage.

Structure	Level	Pattern	Image	Note
TS16: head mesenchyme derived from neural crest	Present	Regionally restricted	1F/G	Expression in cells underneath surface ectoderm in head region.

Structure	Level	Pattern	Image	Note
TS16: head mesenchyme derived from neural crest	Present	Regionally restricted	1H	Expression in clusters of cells close to neural tube caudal to otic vesicle.

Structure	Level	Pattern	Image	Note
TS16: trunk mesenchyme derived from neural crest	Present	Regionally restricted	1I	Expression in individual cells in dorsal midline of trunk neural tube.

Structure	Level	Pattern	Image	Note
TS16: tail mesenchyme derived from neural crest	Absent		1K

Structure	Level	Pattern	Image	Note
TS16: head mesenchyme derived from neural crest	Present	Regionally restricted	1L	Expression in cells in mesenchyme at forebrain-midbrain and midbrain-hindbrain boundaries.
TS16: optic cup outer layer	Present	Regionally restricted	1L	Expression in presumptive retinal pigmented epithelium.

Structure	Level	Pattern	Image	Note
TS16: tail mesenchyme derived from neural crest	Present			In sacral region, expression first observed at 33-34 somite stage.

Structure	Level	Pattern	Image	Note
TS18: head mesenchyme derived from neural crest	Present	Single cells	1M	Expression in single cells in dorso-lateral pathway caudal to otic vesicle.

Structure	Level	Pattern	Image	Note
TS18: trunk mesenchyme derived from neural crest	Present	Single cells	1N	Expression in single cells in trunk area. More numerous that at earlier stages.

Structure	Level	Pattern	Image	Note
TS18: tail mesenchyme derived from neural crest	Present	Single cells	1O	Expression in single cells in sacral area.

Structure	Level	Pattern	Image	Note
TS16: neural crest	Present	Single cells	2A	Expression in single cell in dorsal midline of trunk neural tube - directly overlays neural tube just underneath surface ectoderm.

Structure	Level	Pattern	Image	Note
TS20: tail mesenchyme derived from neural crest	Present	Regionally restricted	2B	Expression in increased numbers of cells in close association with otic vesicle. Authors report Mtif expression ovarlapping with that of Dct.
TS20: mesenchyme derived from neural crest	Present	Regionally restricted	2B	In the trunk, authors report overlap of Mtif and Dct expression in majority of more lateral cells, but not in those closer to dorsal midline.
TS20: dorsal root ganglion	Absent		2B

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme derived from neural crest	Present	Regionally restricted	3A	Expression in increased numbers of cells in close association with otic vesicle. Authors report Mtif expression ovarlapping with that of Dct.

Structure	Level	Pattern	Image	Note
TS19: trunk mesenchyme derived from neural crest	Present	Regionally restricted	3B	In the trunk, authors report overlap of Mtif and Dct expression in majority of more lateral cells, but not in those closer to dorsal midline.

Structure	Level	Pattern	Image	Note
TS19: tail mesenchyme derived from neural crest	Present	Single cells	3C	Expression just beginning in sacral region. Authors report single labeled cells close to dorsal midline. Overlap of Mitf and Dct expression away from midline.

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme derived from neural crest	Present	Single cells	3D Mitf	Expression in rare cells; authors report a reduced number of expressing cells compared with wild-type embryos.

Structure	Level	Pattern	Image	Note
TS19: trunk mesenchyme derived from neural crest	Present	Single cells	3E Mitf	Expression in rare cells; authors report a reduced number of expressing cells compared with wild-type embryos.

Structure	Level	Pattern	Image	Note
TS19: tail mesenchyme derived from neural crest	Present	Single cells	3F Mitf	Expression in rare cells; authors report a reduced number of expressing cells compared with wild-type embryos.

Structure	Level	Pattern	Image	Note
TS16: head mesenchyme derived from neural crest	Present	Regionally restricted	5A	Expression in cells in region between otic vesicle and hindbrain neuroepithelium, corresponding to ventro-medial pathway.

Structure	Level	Pattern	Image	Note
TS21: mesenchyme derived from neural crest	Present	Regionally restricted	5B	Expression in increased numbers of cells in close association with otic vesicle.

Structure	Level	Pattern	Image	Note
TS24: cochlear duct	Present	Regionally restricted	5C	Expression in cells in concentrated in a small area representing the presumptive stria vascularis of the future cochlear duct.

Structure	Level	Pattern	Image	Note
TS27: stria vascularis	Absent

Structure	Level	Pattern	Image	Note
TS20: trunk mesenchyme	Present	Regionally restricted	6A	Expresssion in trunk cells beneath surface ectoderm.
TS20: forelimb epithelium	Present	Single cells	6A	In forelimb bud area, expression in cells incorporated in the epithelial layers.

Structure	Level	Pattern	Image	Note
TS24: dermis	Present		6B
TS24: epidermis	Present		6B

Structure	Level	Pattern	Image	Note
TS27: hair follicle	Present		6C

Structure	Level	Pattern	Image	Note
TS28: hair follicle	Present		6D

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle	Weak			Starting at E9.5, weak but specific expression in ventricular walls.

Structure	Level	Pattern	Image	Note
TS21: head mesenchyme	Weak	Single cells		Weak expression in individual cells around cervical muscles and Meckel's cartilage.
TS21: trunk mesenchyme	Weak	Single cells		Weak expression in individual cells around dorsal aorta, heart atrium, atrio-ventricular bulbar cushion.
TS21: hindlimb mesenchyme	Weak	Single cells		Weak expression in hindlimb mesenchyme, particularly around muscle primordia and ossifying bone.

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Weak	Regionally restricted	7A	Weak expression in proximal parts of neuroepithelium of budding optic vesicle.

Structure	Level	Pattern	Image	Note
TS16: optic cup outer layer	Present	Regionally restricted	7B	Expression in outer layer of optic cup - presumptive retinal pigmented epithelium.
TS16: optic cup inner layer	Absent		7B

Structure	Level	Pattern	Image	Note
TS21: pigmented retinal epithelium	Present		7C	Expression in future retinal pigmented epithelial monolayer.
TS21: mesenchyme derived from neural crest	Present	Regionally restricted	7C	Expression in individual, cells behind the retinal pigmented epithelium, presumably neural-crest-derived. Authors state these may developt into choroidal and anterior iris pigment cells and pigment cells of periorbital skin.

Structure	Level	Pattern	Image	Note
TS24: pigmented retinal epithelium	Present		7D
TS24: eye mesenchyme	Present	Regionally restricted	7D	Expression in individual cells in adjacent mesenchyme - presumably derived from neural crest.

Structure	Level	Pattern	Image	Note
TS27: pigmented retinal epithelium	Absent		7E
TS27: hair follicle	Present		7E

Structure	Level	Pattern	Image	Note
TS27: hair follicle	Absent		7G
TS27: iris	Present	Regionally restricted	7G	xpression retained in retinal pigmented epithelium-derived (back) layer of iris.

Structure	Level	Pattern	Image	Note
TS22: pigmented retinal epithelium	Present		9_Mitf wt
TS22: migrating neural crest	Present		9_Mitf wt

Structure	Level	Pattern	Image	Note
TS22: pigmented retinal epithelium	Present		9_Mitf Mi-wh
TS22: migrating neural crest	Absent		9_Mitf Mi-wh

Structure	Level	Pattern	Image	Note
TS22: pigmented retinal epithelium	Present		9_Mitf mi
TS22: migrating neural crest	Absent		9_Mitf mi

Structure	Level	Pattern	Image	Note
TS22: pigmented retinal epithelium	Present		9_Mitf mi-ew
TS22: migrating neural crest	Absent		9_Mitf mi-ew