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Gene Expression Data
Assay Details
Assay
Reference: J:194471 Borensztein M, et al., Myod and H19-Igf2 locus interactions are required for diaphragm formation in the mouse. Development. 2013 Mar;140(6):1231-9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5898419
Gene symbol: Pax7
Gene name: paired box 7
Antibody: Anti-Pax7
Detection system: Secondary antibody
Results
Specimen 3A upper left: embryonic day 15.5 (more )
Note: Double labeled: green - Pax7; red - Laminin. Data was summarized in Fig 3C.
Structure Level Pattern Image Note
TS23: diaphragm Present 3A upper left

Specimen 3A upper right: embryonic day 15.5; Igf2tm1Rob/Igf2+ (more )
Note: Double labeled: green - Pax7; red - Laminin.. Data was summarized in Fig 3C.
Structure Level Pattern Image Note
TS23: diaphragm Present 3A upper right Same as the wild-type.

Specimen 3A lower left: embryonic day 15.5; Myod1tm1Jae/Myod1tm1Jae (more )
Note: Double labeled: green - Pax7; red - Laminin. Data was summarized in Fig 3C.
Structure Level Pattern Image Note
TS23: diaphragm Present 3A lower left Increased number of positive cells with respect to the number of fibers.

Specimen 3A lower right: embryonic day 15.5; Igf2tm1Rob/Igf2+, Myod1tm1Jae/Myod1tm1Jae (more )
Note: Double labeled: green - Pax7; red - Laminin. Data was summarized in Fig 3C.
Structure Level Pattern Image Note
TS23: diaphragm Present 3A lower right Increased number of positive cells with respect to the number of fibers.

Specimen 3B upper: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: diaphragm Present 3B upper

Specimen 3B lower: embryonic day 15.5; Igf2tm1Rob/Igf2+, Myod1tm1Jae/Myod1tm1Jae (more )
Structure Level Pattern Image Note
TS23: diaphragm Present 3B lower Increased number of positive cells with respect to the number of fibers.

Specimen S4A upper left: embryonic day 13.5 (more )
Note: Double labeled: green - Pax7; red - Laminin.
Structure Level Pattern Image Note
TS21: diaphragm Present S4A upper left

Specimen S4A upper right: embryonic day 13.5; Igf2tm1Rob/Igf2+ (more )
Note: Double labeled: green - Pax7; red - Laminin.
Structure Level Pattern Image Note
TS21: diaphragm Present S4A upper right Same as the wild-type.

Specimen S4A lower left: embryonic day 13.5; Myod1tm1Jae/Myod1tm1Jae (more )
Note: Double labeled: green - Pax7; red - Laminin.
Structure Level Pattern Image Note
TS21: diaphragm Present S4A lower left Same as the wild-type.

Specimen S4A lower right: embryonic day 13.5; Igf2tm1Rob/Igf2+, Myod1tm1Jae/Myod1tm1Jae (more )
Note: Double labeled: green - Pax7; red - Laminin.
Structure Level Pattern Image Note
TS21: diaphragm Present S4A lower right Same as the wild-type.

Specimen S4B upper left: embryonic day 18.5 (more )
Note: Data was summarized in Fig 3D.
Structure Level Pattern Image Note
TS26: diaphragm Present S4B upper left

Specimen S4B upper right: embryonic day 18.5; Igf2tm1Rob/Igf2+ (more )
Note: Data was summarized in Fig 3D.
Structure Level Pattern Image Note
TS26: diaphragm Present S4B upper right Same as the wild-type.

Specimen S4B lower left: embryonic day 18.5; Myod1tm1Jae/Myod1tm1Jae (more )
Note: Data was summarized in Fig 3D.
Structure Level Pattern Image Note
TS26: diaphragm Present S4B lower left Increased numbers of positive cells with the same thickness of the wild-type diaphragm.

Specimen S4B lower right: embryonic day 18.5; Igf2tm1Rob/Igf2+, Myod1tm1Jae/Myod1tm1Jae (more )
Note: Data was summarized in Fig 3D.
Structure Level Pattern Image Note
TS26: diaphragm Present S4B lower right A 4-fold increase in positive cells with thinner diaphragm compared with wild-type.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory