Nefm Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:236098 Shin J, et al., Impaired neurodevelopment by the low complexity domain of CPEB4 reveals a convergent pathway with neurodegeneration. Sci Rep. 2016 Jul 06;6:29395
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5897359
Gene symbol:	Nefm
Gene name:	neurofilament, medium polypeptide

Antibody:	anti-neurofilament clone 2H3
Detection system:	Secondary antibody coupled to fluorescein

Results

1B Neurofilament ©

Specimen 1B: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: dorsal root ganglion	Present		1B Neurofilament
TS27: spinal cord	Present		1B Neurofilament

3A E14.5 Cpeb4+/+ ©

Specimen 3A E14.5 +/+: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: phrenic nerve	Present		3A E14.5 Cpeb4+/+

3A E14.5 Cpeb4GT/GT ©

Specimen 3A E14.5 GT/GT: embryonic day 14.5; Cpeb4^{tm1a(EUCOMM)Wtsi}/Cpeb4^{tm1a(EUCOMM)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS22: phrenic nerve	Present		3A E14.5 Cpeb4GT/GT

3A E15.5 Cpeb4+/+ ©

Specimen 3A E15.5 +/+: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: phrenic nerve	Present		3A E15.5 Cpeb4+/+

3A E15.5 Cpeb4GT/GT ©

Specimen 3A E15.5 GT/GT: embryonic day 15.5; Cpeb4^{tm1a(EUCOMM)Wtsi}/Cpeb4^{tm1a(EUCOMM)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS23: phrenic nerve	Present		3A E15.5 Cpeb4GT/GT

3A E17.5 Cpeb4+/+ ©

Specimen 3A E17.5 +/+: embryonic day 17.5 (more

)

Structure	Level	Pattern	Image	Note
TS25: phrenic nerve	Present		3A E17.5 Cpeb4+/+

3A E17.5 CpebGT/GT ©

Specimen 3A E17.5 GT/GT: embryonic day 17.5; Cpeb4^{tm1a(EUCOMM)Wtsi}/Cpeb4^{tm1a(EUCOMM)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS25: phrenic nerve	Present		3A E17.5 CpebGT/GT

3A E18.5 Cpeb4+/+ ©

Specimen 3A E18.5 +/+: embryonic day 18.5 (more

)

Structure	Level	Pattern	Image	Note
TS26: phrenic nerve	Present		3A E18.5 Cpeb4+/+

3A E18.5 Cpeb4GT/GT ©

Specimen 3A E18.5 GT/GT: embryonic day 18.5; Cpeb4^{tm1a(EUCOMM)Wtsi}/Cpeb4^{tm1a(EUCOMM)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS26: phrenic nerve	Present		3A E18.5 Cpeb4GT/GT	In this mutant axons are thinner and have few arbors.

3D Cpeb4 +/+ left panel ©

3D Cpeb4+/+ right panel ©

Specimen 3D Cpeb4+/+: postnatal day 0 (more

)
Note: Double labeled: green - Nefm; red - alpha-bungarotoxin.

Structure	Level	Pattern	Image	Note
TS27: phrenic nerve	Present		3D Cpeb4+/+ right panel , 3D Cpeb4 +/+ left panel

Specimen 3D Cpeb4GT/GT: postnatal day 0; Cpeb4^{tm1a(EUCOMM)Wtsi}/Cpeb4^{tm1a(EUCOMM)Wtsi} (more

)
Note: Double labeled: green - Nefm; red - alpha-bungarotoxin.

Structure	Level	Pattern	Image	Note
TS27: phrenic nerve	Present		3D Cpeb4GT/GT right panel , 3D Cpeb4GT/GT left panel

3E Cpeb4+/+ ©

Specimen 3E Cpeb4+/+: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Regionally restricted	3E Cpeb4+/+	Expression is in the nerves in the forelimb.
TS20: nerve	Present	Regionally restricted	3E Cpeb4+/+	Expression is in the nerves in the forelimb.

Specimen 3E Cpeb4GT/GT: embryonic day 12.5; Cpeb4^{tm1a(EUCOMM)Wtsi}/Cpeb4^{tm1a(EUCOMM)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Regionally restricted	3E Cpeb4GT/GT	Expression in the nerves in the forelimb is the same as in wild-type.
TS20: nerve	Present	Regionally restricted	3E Cpeb4GT/GT	Expression in the nerves in the forelimb is the same as in wild-type.

S2B Cpeb4+/+ ©

Specimen S2B Cpeb4+/+: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: trigeminal nerve ophthalmic division	Present		S2B Cpeb4+/+

Specimen S2B Cpeb4GT/GT: embryonic day 12.5; Cpeb4^{tm1a(EUCOMM)Wtsi}/Cpeb4^{tm1a(EUCOMM)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS20: trigeminal nerve ophthalmic division	Present		S2B Cpeb4GT/GT	Expression in this mutant is the same as in wil-type.

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