Probe:	cDNA-D
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS23: cricoid cartilage	Present	Regionally restricted	1A/B	Expression was confined to the mesenchyme surrounding the condensing cartilages, and stronger on the ventral side.
TS23: thyroid cartilage	Present	Regionally restricted	1A/B	Expression was confined to the mesenchyme surrounding the condensing cartilages, and stronger on the ventral side.
TS23: respiratory system cartilage	Present	Regionally restricted	1A/B	Expressed in tracheal cartilage. Expression was confined to the mesenchyme surrounding the condensing cartilages, and stronger on the ventral side.

Structure	Level	Pattern	Image	Note
TS21: forelimb joint	Present	Regionally restricted	3A/B	Expressed in the shoulder joint.
TS21: forelimb mesenchyme	Present	Regionally restricted	3A/B	Expressed in the ligament attached to the humerus.

Structure	Level	Pattern	Image	Note
TS22: hindlimb joint	Present	Regionally restricted	3C/D	Expressed in the hip joint region between the head of the femur and the cup-shaped acetabulum of the innominate bone.
TS22: appendicular skeleton	Present	Regionally restricted	3C/D	Expressed in the perichondrium surrounding the cartilage primordia of the iliac bone.

Structure	Level	Pattern	Image	Note
TS22: appendicular skeleton	Present	Regionally restricted	3E/F	Expressed in the perichondrium surrounding the cartilage primordia of the pubic bone and the ischial bone.
TS22: upper leg mesenchyme	Strong	Regionally restricted	3E/F	Expressed in ligaments attached to the femur.
TS22: hindlimb muscle	Strong	Regionally restricted	3E/F	Expressed in a forming adductor muscle initiating at the pubic bone.
TS22: perichondrium	Present		3E/F
TS22: lower leg mesenchyme	Present	Regionally restricted	3E/F	Expressed in mesenchyme surrounding the tibia and fibula.
TS22: genital tubercle	Present		3E/F

Structure	Level	Pattern	Image	Note
TS21: perichondrium	Present		3G/H
TS21: lower leg mesenchyme	Present	Regionally restricted	3G/H	Expressed in mesenchyme surrounding the tibia and fibula.

Structure	Level	Pattern	Image	Note
TS23: genital tubercle mesenchyme	Strong	Regionally restricted	3I/J	Expressed in the mesenchyme of the labioscrotal swellings.