Antibody:	mUBPy antibody
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong	Regionally restricted	3A	Expressed in the wall of the telencephalic vesicles.

Structure	Level	Pattern	Image	Note
TS17: future neurohypophysis	Present	Regionally restricted	3B	Expressed in the hypothalamus.
TS17: diencephalon ventricular layer	Strong	Regionally restricted	3B	Expressed in the periventricular cell layer of the third ventricle.
TS17: diencephalon	Weak	Regionally restricted	3B	Expressed in the roof of the third ventricle, the ventral and dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Moderate	Regionally restricted	3C	Expressed in the metencephalic and the myelencephalic parts of rhomboencephalon. Low expression in the alar plate of myelencephalon.
TS17: diencephalon	Moderate	Regionally restricted	3C	Expressed in the wall and the floor of the diencephalon.
TS17: midbrain	Moderate	Regionally restricted	3C	Expressed in the ependymal layer.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Moderate	Regionally restricted	3D	Expressed in the median sulcus of the fourth ventricle.
TS17: hindbrain mantle layer	Present		3D

Structure	Level	Pattern	Image	Note
TS20: corpus striatum	Strong		3E

Structure	Level	Pattern	Image	Note
TS20: thalamus	Strong	Regionally restricted	3F	Expressed in dorsal and ventral thalamus.
TS20: diencephalon	Strong	Regionally restricted	3F	Expressed in the roof and the floor of the third ventricle.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Strong	Regionally restricted	3G	Expressed in the caudal hypothalamus.

Structure	Level	Pattern	Image	Note
TS20: hindbrain mantle layer	Strong		3H
TS20: medulla oblongata alar plate	Strong		3H

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Moderate	Regionally restricted	3I	Expressed in the roof of the fourth ventricle.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Moderate	Regionally restricted	4A	Expressed in the periventricular layer of the third ventricle.

Structure	Level	Pattern	Image	Note
TS22: thalamus	Moderate	Regionally restricted	4B	Expressed in the ventral thalamus.
TS22: neocortex	Moderate		4B

Structure	Level	Pattern	Image	Note
TS24: hindbrain mantle layer	Strong	Regionally restricted	4C	Expressed in themantle layer of the fourth ventricle.
TS24: hindbrain marginal layer	Weak		4C	Very low expression.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Strong	Regionally restricted	4D	Expressed in the periventricular cell layer and the roof of the third ventricle. A moderate expression in the floor of the third ventricle.

Structure	Level	Pattern	Image	Note
TS24: hypothalamus	Weak	Regionally restricted	4E	Expressed in the caudal hypothalamus.

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Weak	Regionally restricted	4F	Expressed in the wall of the telencephalic vesicle. A very low immunostaining is present in the external region of the telencephalic vesicle.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Strong	Regionally restricted	5A	Expressed in the motor cortex. A weak immunostaining is also present in the external region of the cortex.

Structure	Level	Pattern	Image	Note
TS27: perirhinal cortex	Strong		5B

Structure	Level	Pattern	Image	Note
TS27: insular cortex	Strong		5C	Expressed in the granular and agranular insular cortex.

Structure	Level	Pattern	Image	Note
TS27: visual cortex	Moderate	Regionally restricted	5D	Expressed in the primary visual cortex.
TS27: frontal cortex	Present	Regionally restricted	5D	Expressed in the lateral orbital cortex.

Structure	Level	Pattern	Image	Note
TS27: hippocampus CA1	Moderate	Single cells	5E
TS27: hippocampus CA2	Moderate	Single cells	5E
TS27: hippocampus CA3	Moderate	Single cells	5E

Structure	Level	Pattern	Image	Note
TS28: amygdalohippocampal area	Moderate		5F

Structure	Level	Pattern	Image	Note
TS28: pallidum	Present	Regionally restricted	5G	Expressed in the ventral pallidum.

Structure	Level	Pattern	Image	Note
TS28: entorhinal cortex	Present	Scattered	5H

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Moderate		5I

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	6C , 6A	Expressed in the caudal hypothalamus. Expressed in neurons.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata alar plate	Present		6F , 6D	Expressed in astrocytes.

Structure	Level	Pattern	Image	Note
TS22: hindbrain mantle layer	Present	Regionally restricted	6I , 6G	Expressed in the mantle layer of the fourth ventricle. Expressed in dopaminergic neurons.
TS22: midbrain	Present	Regionally restricted	6I , 6G	Expressed in dopaminergic neurons of the pars compacta of the substantia nigra.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	7A , 7C	Expressed in the metencephalic parts of rhomboencephalon. Expressed in some subsets of neurons.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present		7D , 7F	Expressed in some subsets of neurons.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present		7I , 7G	Expressed in some subsets of neurons.

Structure	Level	Pattern	Image	Note
TS17: lens vesicle	Strong	Regionally restricted	8A	Expressed in the anterior wall, elongated cells of the posterior wall, and moderate expression in equatorial region.
TS17: eye surface ectoderm	Present	Regionally restricted	8A	Expressed in the ectoderm and the surface epithelium of the cornea.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present		8B	Expressed in the ependymal cells of the otic vesicle.

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Strong	Widespread	8C	More intense expression in the primary and secondary lens fibers.
TS20: cornea	Absent		8C

Structure	Level	Pattern	Image	Note
TS20: semicircular canal epithelium	Present		8D

Structure	Level	Pattern	Image	Note
TS20: endolymphatic sac epithelium	Strong		8E
TS20: semicircular canal epithelium	Strong		8E

Structure	Level	Pattern	Image	Note
TS22: lens fiber	Strong		8F	Expressed in the primary lens, and low expression in the secondary lens fibres.
TS22: cornea epithelium	Moderate		8F	Expressed in the surface epithelium and mesothelial layer.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present	Scattered	8G	Expressed in the ependymal cells of the lumen of the primitive olfactory cavity.

Structure	Level	Pattern	Image	Note
TS24: lens fiber	Present	Regionally restricted	8H	Expressed in the central region of the primary lens fibres.
TS24: cornea epithelium	Weak	Regionally restricted	8H	Expressed in the surface epithelium.

Structure	Level	Pattern	Image	Note
TS22: macula of vestibular component	Present		8I
TS22: saccule epithelium	Present		8I

Structure	Level	Pattern	Image	Note
TS24: cochlea	Strong		8L

Structure	Level	Pattern	Image	Note
TS24: olfactory epithelium	Strong		8M , 8N	Expressed in the olfactory epithelium of the nasal cavity and in the ependymal cells of the lumen of the olfactory cavity.

Structure	Level	Pattern	Image	Note
TS13: future brain	Absent

Structure	Level	Pattern	Image	Note
TS17: nasal pit epithelium	Present	Regionally restricted		Expressed along the lateral region.
TS17: olfactory placode	Present	Regionally restricted		Expressed along the lateral region.

Structure	Level	Pattern	Image	Note
TS20: corpus striatum	Moderate	Regionally restricted		Expressed in the caudate nucleus.
TS20: epithalamus	Moderate
TS20: neocortex	Moderate	Regionally restricted		Expressed in the roof.
TS20: midbrain	Moderate	Regionally restricted		Expressed in the wall and roof.
TS20: pons	Present	Scattered		Expressed in in the basal plate and the tegmentum.
TS20: nasal epithelium	Present	Regionally restricted		Expressed along the lateral region.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted		Decreased expression in the floor.
TS22: hypothalamus	Present	Regionally restricted		Decreased expression in the caudal hypothalamus.
TS22: medulla oblongata alar plate	Present			Decreased expression.
TS22: midbrain floor plate	Present			Decreased expression.
TS22: midbrain lateral wall	Present			Decreased expression.
TS22: hindbrain	Present	Regionally restricted		Expressed in the caudal part of the fourth ventricle.
TS22: midbrain	Present	Regionally restricted		Decreased expression in the ependymal layer.

Structure	Level	Pattern	Image	Note
TS24: midbrain	Weak	Regionally restricted		Expressed in the roof.
TS24: thalamus	Present	Regionally restricted		Decreased expression in the ventral and dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS26: brain	Absent

Structure	Level	Pattern	Image	Note
TS27: brain	Absent

Structure	Level	Pattern	Image	Note
TS27: piriform cortex	Strong
TS27: primary somatosensory cortex	Strong
TS27: cingulate cortex	Moderate
TS27: insular cortex	Moderate
TS27: cerebral cortex	Moderate	Regionally restricted		Expressed in the external layers.
TS27: midbrain	Moderate	Regionally restricted		Expressed in the deep mesencephalic nucleus.
TS27: caudate-putamen	Moderate
TS27: hypothalamus lateral zone	Moderate
TS27: substantia nigra pars compacta	Moderate
TS27: ventral tegmental area	Moderate
TS27: trigeminal nucleus	Moderate	Regionally restricted		Expressed in the oral part.

Structure	Level	Pattern	Image	Note
TS28: basal forebrain	Moderate	Regionally restricted		Expressed in the horizontal limb of the diagonal band.
TS28: inferior olivary nucleus	Moderate
TS28: nucleus of lateral olfactory tract	Moderate
TS28: olfactory tubercle	Moderate
TS28: anterior amygdaloid area	Present	Scattered
TS28: anterior thalamic group	Present	Scattered
TS28: arcuate nucleus	Present	Scattered
TS28: central medial thalamic nucleus	Present	Scattered
TS28: dorsomedial hypothalamic nucleus	Present	Scattered
TS28: hippocampus stratum lacunosum	Present	Scattered
TS28: lateral mammillary nucleus	Present	Scattered
TS28: substantia nigra pars lateralis	Present	Scattered
TS28: supramammillary nucleus	Present	Scattered
TS28: ventromedial hypothalamic nucleus	Present	Scattered
TS28: pallidum	Present	Scattered		Expressed in the ventral pallidum.
TS28: lateral thalamic group	Present	Scattered		Expressed in the laterodorsal thalamic nucleus.
TS28: midbrain	Present	Regionally restricted		Decreased expression in the deep mesencephalic nucleus.
TS28: cerebral cortex	Present	Regionally restricted		Decreased expression in different regions of the cortex.
TS28: caudate-putamen	Present			Increased expression.
TS28: hippocampus CA1	Present			Increased expression.
TS28: substantia nigra	Present	Regionally restricted		Increased expression in the compact part.
TS28: trigeminal V spinal sensory nucleus	Present	Regionally restricted		Increased expression in the oral part.