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Gene Expression Data
Assay Details
Assay
Reference: J:200255 Pasque V, et al., Histone variant macroH2A marks embryonic differentiation in vivo and acts as an epigenetic barrier to induced pluripotency. J Cell Sci. 2012 Dec 15;125(Pt 24):6094-104
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5882066
Gene symbol: Nanog
Gene name: Nanog homeobox
Antibody: anti-Nanog (eBioMLC-51, 14-5761-80)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1A: embryonic day 3.5 (more )
Note: Triple labeled: green - Nanog; red - Macroh2a1 (assay MGI:5882067); blue - Pou5f1 (assay MGI:5882065).
Structure Level Pattern Image Note
TS5: inner cell mass Present 1A Nanog , 1A macroH2A.1/Nanog/Oct4
Specimen 1B: embryonic day 4.5 (more )
Note: Triple labeled: green - Nanog; red - Macroh2a1 (assay MGI:5882067); blue - Pou5f1 (assay MGI:5882065).
Structure Level Pattern Image Note
TS6: blastocyst Present 1B macroH2A.1/Nanog/Oct4 , 1B Nanog
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory