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Gene Expression Data
Assay Details
Assay
Reference: J:76826 Zuniga A, et al., Mouse Twist is required for fibroblast growth factor-mediated epithelial-mesenchymal signalling and cell survival during limb morphogenesis. Mech Dev. 2002 Jun;114(1-2):51
Assay type: RNA in situ
MGI Accession ID: MGI:5881825
Gene symbol: Fgf8
Gene name: fibroblast growth factor 8
Probe: Fgf8 cDNA13
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 3K: embryonic day 9.75 (more )
Note: 27-28 somites
Structure Level Pattern Image Note
TS15: forelimb bud presumptive apical ectodermal ridge Present 3K
Specimen 3L: embryonic day 9.75; Twist1tm1Bhr/Twist1tm1Bhr (more )
Note: 27-28 somites
Structure Level Pattern Image Note
TS15: forelimb bud presumptive apical ectodermal ridge Present Regionally restricted 3L Expression is more anterior restricted in the apical ectodermal ridge of mutant forelimb buds by E9.75.
Specimen 3M: embryonic day 10.25 (more )
Note: 33-35 somites
Structure Level Pattern Image Note
TS16: hindlimb bud ectoderm Present Regionally restricted 3M Authors report expression in the apical ectodermal ridge.
Specimen 3N: embryonic day 10.25; Twist1tm1Bhr/Twist1tm1Bhr (more )
Note: 33-35 somites
Structure Level Pattern Image Note
TS16: hindlimb bud ectoderm Present Regionally restricted 3N Authors report expression is more anterior restricted in the apical ectodermal ridge of mutant hindimb buds by E10.25 compared with wild-type.
Specimen 3O: embryonic day 10.25; Twist1tm1Bhr/Twist1tm1Bhr (more )
Note: 33-35 somites
Structure Level Pattern Image Note
TS16: forelimb bud apical ectodermal ridge Ambiguous 3O Authors report expression is almost completely lost from the apical ectodermal ridge of forelimb buds.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory