Gene Expression Data
Assay Details
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Assay
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Reference:
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J:76826
Zuniga A, et al., Mouse Twist is required for fibroblast growth factor-mediated epithelial-mesenchymal signalling and cell survival during limb morphogenesis. Mech Dev. 2002 Jun;114(1-2):51
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5881823
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Gene symbol:
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Fgfr1
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Gene name:
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fibroblast growth factor receptor 1
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Probe:
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Fgfr1 cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3A:
embryonic day 9.75
(more  )
Note: 27-28 somites
Specimen
3A:
(close  )
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Genetic Background:
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involves: C57BL/6J * CD-1
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Age:
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embryonic day 9.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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27-28 somites
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J * CD-1b
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Mutant Allele(s):
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Twist1tm1Bhr/Twist1tm1Bhr
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Age:
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embryonic day 9.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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27 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud mesenchyme |
Weak |
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3B
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Expression is significantly reduced in the mutant forelimb bud by E9.75 (27 somites).
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Specimen
3C:
embryonic day 10.25
(more )
Note: 33-35 somites
Specimen
3C:
(close )
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Genetic Background:
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involves: C57BL/6J * CD-1
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Age:
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embryonic day 10.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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33-35 somites
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J * CD-1b
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Mutant Allele(s):
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Twist1tm1Bhr/Twist1tm1Bhr
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Age:
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embryonic day 10.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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33-35 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: hindlimb bud mesenchyme |
Weak |
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3D
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Expression levels remain low in older mutant hindlimb bud.
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| TS16: somite |
Weak |
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3D
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Expression is reduced in somites of mutant embryos compared with wild-type.
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Specimen
3E:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J * CD-1b
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Mutant Allele(s):
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Twist1tm1Bhr/Twist1tm1Bhr
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Age:
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embryonic day 10.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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33-35 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: hindlimb bud mesenchyme |
Weak |
Regionally restricted |
3E
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Expression only detected in the distal-most mesenchme.
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| TS16: somite |
Weak |
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3E
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Expression is reduced in somites of mutant embryos compared with wild-type.
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Analysis Tools
