Probe:	Raldh1 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: bronchus epithelium	Strong
TS20: lens vesicle epithelium	Strong
TS20: trachea epithelium	Strong

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	1A/B	Specific expression in ventral prospective dopaminergic midbrain cells.
TS22: intestine mesenchyme	Strong	Regionally restricted	1A/B	Strong expression in outer intestinal mesenchyme.
TS22: stomach epithelium	Weak	Regionally restricted	1A/B	Weaker expression in stomach left wall epithelium.
TS22: bronchus epithelium	Strong		1A/B
TS22: nasal cavity respiratory epithelium	Strong		1A/B
TS22: salivary gland epithelium	Strong		1A/B
TS22: trachea epithelium	Strong		1A/B
TS22: olfactory epithelium	Strong	Regionally restricted	1A/B	Strong expression in part of the olfactory epithelium.
TS22: metanephros	Present	Regionally restricted	1A/B	Expression in few cells of the kidney, which may correspond to branches of the ureteric bud.
TS22: liver	Weak		1A/B

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	1D/E	Specific expression in ventral prospective dopaminergic midbrain cells.
TS22: intestine mesenchyme	Strong	Regionally restricted	1D/E	Strong expression in outer intestinal mesenchyme.
TS22: nasal cavity respiratory epithelium	Strong		1D/E
TS22: olfactory epithelium	Strong	Regionally restricted	1D/E	Strong expression in part of the olfactory epithelium.

Structure	Level	Pattern	Image	Note
TS24: esophagus epithelium	Weak		2A/B
TS24: esophagus mesenchyme	Weak		2A/B
TS24: lung parenchyma	Moderate	Widespread	2A/B
TS24: liver	Weak		2A/B
TS24: metanephros	Moderate	Regionally restricted	2A/B	Expression in few cells of the kidney, which may correspond to branches of the ureteric bud.

Structure	Level	Pattern	Image	Note
TS24: small intestine epithelium	Present		2E/F
TS24: large intestine epithelium	Absent		2E/F

Structure	Level	Pattern	Image	Note
TS27: intestinal villus	Present	Regionally restricted		Expression localized to base of intestinal glands and stem of intestinal villi.

Structure	Level	Pattern	Image	Note
TS28: intestinal villus	Present	Regionally restricted		Expression localized to the stem of intestinal villi in fully differentiated intestine.

Structure	Level	Pattern	Image	Note
TS28: metanephros	Present	Regionally restricted	2I/J
TS28: collecting duct	Present		2I/J
TS28: adrenal cortex	Present	Regionally restricted	2I/J

Structure	Level	Pattern	Image	Note
TS28: adrenal gland zona reticularis	Present		2J inset

Structure	Level	Pattern	Image	Note
TS28: outer renal medulla	Present		2L

Structure	Level	Pattern	Image	Note
TS28: male accessory gland	Strong	Regionally restricted	2M/N	Strong expression in epithelia of developing sex glands.

Structure	Level	Pattern	Image	Note
TS15: heart	Present	Regionally restricted	3A/B
TS15: visceral pericardium	Present		3A/B	Expression in the developing epicardial layer.
TS15: pharynx epithelium	Present	Regionally restricted	3A/B	Expression in the caudal pharyngeal epithelium.

Structure	Level	Pattern	Image	Note
TS22: visceral pericardium	Ambiguous			Authors report epicardial expression fades away.

Structure	Level	Pattern	Image	Note
TS24: visceral pericardium	Ambiguous		3C/D	Authors report epicardial expression fades away.
TS24: atrioventricular valve	Present		3C/D
TS24: lung parenchyma	Moderate		3C/D

Structure	Level	Pattern	Image	Note
TS24: semilunar valve	Present		3E/F
TS24: lung parenchyma	Present		3E/F
TS24: pulmonary artery	Present	Regionally restricted	3E/F	Expression along the wall of the proximal aorta and pulmonary artery.
TS24: thoracic aorta	Present	Regionally restricted	3E/F	Expression along the wall of the proximal aorta and pulmonary artery.

Structure	Level	Pattern	Image	Note
TS24: nose	Present	Regionally restricted	4A/B	Expression mostly found towards lateral regions of developing turbinates and in developing nasal glands.

Structure	Level	Pattern	Image	Note
TS28: nose	Present	Regionally restricted	4E/F	Expression along the olfactory mesenchyme.

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present		5A/B
TS24: tooth epithelium	Present	Regionally restricted	5A/B	Expression specific to the junction between the oral and dental epithelium.
TS24: oral epithelium	Present	Widespread	5A/B
TS24: pharynx epithelium	Present	Widespread	5A/B

Structure	Level	Pattern	Image	Note
TS24: upper jaw molar epithelium	Present	Regionally restricted	5E , 5G	Expression specific to the junction between the oral and dental epithelium.
TS24: oral epithelium	Present	Widespread	5E , 5G
TS24: pharynx epithelium	Present	Widespread	5E , 5G

Structure	Level	Pattern	Image	Note
TS24: lower jaw incisor dental papilla	Present	Regionally restricted	5H , 5F	Expression towards the base of the incisor papilla mesenchyme.

Structure	Level	Pattern	Image	Note
TS28: upper jaw incisor dental papilla	Present	Regionally restricted	5M/N	Expression in basal/lateral areas of the incisor papilla mesenchyme.

Structure	Level	Pattern	Image	Note
TS28: molar dental papilla	Absent

Structure	Level	Pattern	Image	Note
TS28: midbrain	Present	Regionally restricted	6A/B	Expression in specific ventral midbrain cells corresponding to dopaminergic progenitors.
TS28: brain meninges	Present		6A/B

Structure	Level	Pattern	Image	Note
TS28: midbrain	Strong	Regionally restricted	6E	Strong expression in ventral mesencephalic cells, including the substantia nigra.
TS28: substantia nigra	Strong		6E

Structure	Level	Pattern	Image	Note
TS28: midbrain	Strong	Regionally restricted	6G	Strong expression in ventral mesencephalic cells, including the substantia nigra.
TS28: substantia nigra	Strong		6G
TS28: brain nucleus	Weak	Regionally restricted	6G	Weaker expression in dorsal mesencephalic, pontine, medulla and diencephalic nuclei.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	6H	Expression in cells of the deep layers of the cerebral cortex.
TS28: brain nucleus	Weak	Regionally restricted	6H	Weaker expression in dorsal mesencephalic, pontine, medulla and diencephalic nuclei.

Structure	Level	Pattern	Image	Note
TS28: Harderian gland	Present
TS28: conjunctival sac	Present