Probe:	Tet1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS22: mesenchymal layer of pelvic urethra	Present		3A

Structure	Level	Pattern	Image	Note
TS25: mesenchymal layer of pelvic urethra of male	Present		3B	Staining intensity increased from E14.5
TS25: epithelium of pelvic urethra of male	Absent		3B

Structure	Level	Pattern	Image	Note
TS28: prostate gland	Present	Regionally restricted	3C	Expression was detected in prostatic buds.

Structure	Level	Pattern	Image	Note
TS22: mesenchymal layer of pelvic urethra	Present		3D

Structure	Level	Pattern	Image	Note
TS25: mesenchymal layer of pelvic urethra of female	Present		3E	Staining intensity increased from E14.5.
TS25: epithelium of pelvic urethra of female	Present	Spotted	3E

Structure	Level	Pattern	Image	Note
TS28: lower urogenital tract of female	Ambiguous		3F

Structure	Level	Pattern	Image	Note
TS22: epithelium of pelvic urethra	Absent
TS22: pelvic urethra	Moderate	Non-Uniform		Medium expression was detected in the muscularis propria. Low expression was detected in the lamina propria, muscularis mucosa, and submucosa.
TS22: developing vasculature of pelvic urethra	Absent
TS22: mesenchyme derived from neural crest	Absent			No staining was detected in the neural crest derivatives of the pelvic urethra.
TS22: epithelium of nephric duct of male	Moderate
TS22: mesenchyme of nephric duct of male	Moderate

Structure	Level	Pattern	Image	Note
TS22: epithelium of pelvic urethra	Weak
TS22: pelvic urethra	Moderate	Regionally restricted		Medium expression was detected in the lamina propria, muscularis mucosa, submucosa, and muscularis propria.
TS22: developing vasculature of pelvic urethra	Absent
TS22: mesenchyme derived from neural crest	Absent			No staining was detected in the neural crest derivatives of the pelvic urethra.
TS22: epithelium of paramesonephric duct of female	Moderate
TS22: mesenchyme of paramesonephric duct of female	Moderate

Structure	Level	Pattern	Image	Note
TS25: basal cell layer of pelvic urethra of male	Absent
TS25: epithelium of pelvic urethra of male	Absent
TS25: intermediate cell layer of pelvic urethra of male	Absent
TS25: superficial cell layer of pelvic urethra of male	Absent
TS25: lamina propria of pelvic urethra of male	Moderate
TS25: mesenchymal pad of pelvic urethra of male	Moderate
TS25: muscularis mucosa of pelvic urethra of male	Moderate
TS25: submucosa of pelvic urethra of male	Moderate
TS25: muscle layer of pelvic urethra of male	Weak
TS25: presumptive neural crest derivative of pelvic urethra of male	Absent
TS25: vasculature of pelvic urethra of male	Absent
TS25: epithelium of seminal vesicle	Weak
TS25: muscle of seminal vesicle	Weak
TS25: vasculature of seminal vesicle	Absent
TS25: seminal vesicle	Weak	Regionally restricted		Low expression was detected in the mesenchymal layer. No staining was detected in the adventitia.
TS25: epithelium of ejaculatory duct	Weak
TS25: muscle of ejaculatory duct	Weak
TS25: vasculature of ejaculatory duct	Absent
TS25: ejaculatory duct	Moderate	Regionally restricted		Medium expression was detected in the mesenchymal layer.
TS25: prostate epithelium	Absent
TS25: peri-prostatic mesenchyme	Moderate

Structure	Level	Pattern	Image	Note
TS25: basal cell layer of pelvic urethra of female	Moderate
TS25: epithelium of pelvic urethra of female	Moderate
TS25: intermediate cell layer of pelvic urethra of female	Moderate
TS25: superficial cell layer of pelvic urethra of female	Moderate
TS25: lamina propria of pelvic urethra of female	Moderate
TS25: muscularis mucosa of pelvic urethra of female	Moderate
TS25: submucosa of pelvic urethra of female	Moderate
TS25: muscle layer of pelvic urethra of female	Weak
TS25: mesenchymal pad of pelvic urethra of female	Moderate
TS25: presumptive neural crest derivative of pelvic urethra of female	Absent
TS25: vasculature of pelvic urethra of female	Absent
TS25: epithelium of upper vagina	Moderate
TS25: muscle of upper vagina	Moderate
TS25: adventitia of upper vagina	Absent
TS25: vasculature of upper vagina	Absent
TS25: upper vagina	Moderate	Regionally restricted		Medium expression was detected in the mesenchymal layer.
TS25: epithelium of sinovaginal bulb	Moderate
TS25: muscle of sinovaginal bulb	Moderate
TS25: adventitia of sinovaginal bulb	Absent
TS25: vasculature of sinovaginal bulb	Absent
TS25: sinovaginal bulb	Moderate	Regionally restricted		Medium expression was detected in the mesenchymal layer.

Structure	Level	Pattern	Image	Note
TS28: basal cell layer of pelvic urethra of male	Weak
TS28: epithelium of pelvic urethra of male	Weak
TS28: intermediate cell layer of pelvic urethra of male	Absent
TS28: superficial cell layer of pelvic urethra of male	Absent
TS28: lamina propria of pelvic urethra of male	Moderate
TS28: muscularis mucosa of pelvic urethra of male	Moderate
TS28: submucosa of pelvic urethra of male	Moderate
TS28: muscle layer of pelvic urethra of male	Weak
TS28: mesenchymal pad of pelvic urethra of male	Moderate
TS28: presumptive neural crest derivative of pelvic urethra of male	Absent
TS28: vasculature of pelvic urethra of male	Absent
TS28: epithelium of seminal vesicle	Moderate
TS28: muscle of seminal vesicle	Moderate
TS28: vasculature of seminal vesicle	Absent
TS28: seminal vesicle	Moderate	Regionally restricted		Medium expression was detected in the mesenchymal layer. No staining was detected in the adventitia.
TS28: epithelium of ejaculatory duct	Moderate
TS28: muscle of ejaculatory duct	Moderate
TS28: vasculature of ejaculatory duct	Absent
TS28: ejaculatory duct	Moderate	Regionally restricted		Medium expression was detected in the mesenchymal layer.
TS28: peri-prostatic mesenchyme	Moderate
TS28: prostate epithelium	Moderate

Structure	Level	Pattern	Image	Note
TS28: basal cell layer of pelvic urethra of female	Weak
TS28: epithelium of pelvic urethra of female	Weak
TS28: intermediate cell layer of pelvic urethra of female	Absent
TS28: superficial cell layer of pelvic urethra of female	Absent
TS28: lamina propria of pelvic urethra of female	Moderate
TS28: muscularis mucosa of pelvic urethra of female	Moderate
TS28: submucosa of pelvic urethra of female	Moderate
TS28: muscle layer of pelvic urethra of female	Weak
TS28: mesenchymal pad of pelvic urethra of female	Moderate
TS28: presumptive neural crest derivative of pelvic urethra of female	Absent
TS28: vasculature of pelvic urethra of female	Absent
TS28: epithelium of vagina	Moderate
TS28: muscle of vagina	Moderate
TS28: adventitia of vagina	Absent
TS28: vasculature of vagina	Absent
TS28: vagina	Moderate	Regionally restricted		Medium expression was detected in the mesenchymal layer.