Probe:	Nkd1 intron probe
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS19: tail unsegmented mesenchyme	Present	Regionally restricted	3F	Expression pattern described as Phase I: lower expression intensities in the tailbud.

Structure	Level	Pattern	Image	Note
TS19: tail unsegmented mesenchyme	Present	Regionally restricted	3G	Expression pattern described as Phase II: expression evident in middle of presomitic mesoderm in addition to the tailbud.

Structure	Level	Pattern	Image	Note
TS19: tail unsegmented mesenchyme	Present	Regionally restricted	3H	Expression pattern described as Phase III: expression shifts more anteriorly.

Structure	Level	Pattern	Image	Note
TS19: tail unsegmented mesenchyme	Present	Regionally restricted	4A nkd1	Expression in the middle presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS19: tail unsegmented mesenchyme	Present	Regionally restricted	4B nkd1	Expression in the middle presomitic mesoderm; no narrow band of experssion in the anterior presomitc mesoderm.

Structure	Level	Pattern	Image	Note
TS19: tail unsegmented mesenchyme	Present	Regionally restricted	4C nkd1	Expression in the middle presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS19: tail unsegmented mesenchyme	Present	Regionally restricted	4D nkd1

Structure	Level	Pattern	Image	Note
TS19: tail unsegmented mesenchyme	Strong	Regionally restricted	4E nkd1	Strong expression in the middle presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS19: tail unsegmented mesenchyme	Present	Regionally restricted	4F nkd1

Structure	Level	Pattern	Image	Note
TS19: tail unsegmented mesenchyme	Strong	Regionally restricted	5A	Authors state expression is upregulated. Strong expression in middle presomitic mesoderm, and weaker expression in the remaining presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS19: tail unsegmented mesenchyme	Strong	Regionally restricted	5B	Authors state expression is upregulated. Strong expression in middle presomitic mesoderm, and weaker expression in the remaining presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS19: tail unsegmented mesenchyme	Strong	Regionally restricted	5C	Authors state expression is upregulated. Strong expression in middle presomitic mesoderm, and weaker expression in the remaining presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS19: tail unsegmented mesenchyme	Present	Regionally restricted	5D

Structure	Level	Pattern	Image	Note
TS19: tail unsegmented mesenchyme	Present	Regionally restricted	5E

Structure	Level	Pattern	Image	Note
TS17: tail unsegmented mesenchyme	Weak	Regionally restricted	5F	Expression is downregulated in middle presomitic mesoderm and tailbud regions.

Structure	Level	Pattern	Image	Note
TS17: tail unsegmented mesenchyme	Weak		5G	Expression is downregulated in middle presomitic mesoderm and tailbud regions.

Structure	Level	Pattern	Image	Note
TS17: tail unsegmented mesenchyme	Present	Regionally restricted	5H	Expression in anterior presomitic mesoderm of wild-type embryo.

Structure	Level	Pattern	Image	Note
TS17: tail unsegmented mesenchyme	Present		5I

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		5J	Normal expression in neural tube and newly formed somites.
TS17: somite	Present		5J	Normal expression in neural tube and newly formed somites.

Structure	Level	Pattern	Image	Note
TS17: tail unsegmented mesenchyme	Present		5K	Authors state expression levels were unaltered compared with wild type.

Structure	Level	Pattern	Image	Note
TS17: tail unsegmented mesenchyme	Present		5L	Authors state expression levels were unaltered compared with wild type.

Structure	Level	Pattern	Image	Note
TS17: tail unsegmented mesenchyme	Present		5M

Structure	Level	Pattern	Image	Note
TS17: tail unsegmented mesenchyme	Present		5N