Gene Expression Data
Assay Details
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Assay
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Reference:
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J:165754
Watanabe T, et al., Transcription factor CHF1/Hey2 regulates coronary vascular maturation. Mech Dev. 2010 Sep-Dec;127(9-12):418-27
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5828246
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Gene symbol:
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Pecam1
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Gene name:
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platelet/endothelial cell adhesion molecule 1
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Antibody:
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MEC 13.3
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
2a:
embryonic day 15.5
(more  )
Specimen
2a:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
2b:
(close )
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Genetic Background:
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C.Cg-Hey2tm1Mtc
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Mutant Allele(s):
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Hey2tm1Mtc/Hey2tm1Mtc
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
2c:
embryonic day 15.5
(more )
Specimen
2c:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cardiac muscle tissue |
Present |
Regionally restricted |
2c
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Expression in endothelial cells of the myocardial vasculature.
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Specimen
2c inset:
embryonic day 15.5
(more )
Specimen
2c inset:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: heart blood vessel |
Present |
Regionally restricted |
2c inset
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Expression in fine vessels and some larger vessels with stable lumens.
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Specimen
2d:
(close )
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Genetic Background:
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C.Cg-Hey2tm1Mtc
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Mutant Allele(s):
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Hey2tm1Mtc/Hey2tm1Mtc
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cardiac muscle tissue |
Present |
Regionally restricted |
2d
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Expression in endothelial cells of the myocardial vasculature.
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Specimen
2d inset:
(close )
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Genetic Background:
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C.Cg-Hey2tm1Mtc
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Mutant Allele(s):
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Hey2tm1Mtc/Hey2tm1Mtc
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: heart blood vessel |
Present |
Regionally restricted |
2d inset
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Expression in collapsed fine vessels and dysmorphic larger vessels.
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Specimen
3a:
embryonic day 18.5
(more )
Specimen
3a:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: coronary artery |
Present |
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3a
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Expression in well-developed coronary arteries.
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Specimen
3b:
(close )
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Genetic Background:
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C.Cg-Hey2tm1Mtc
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Mutant Allele(s):
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Hey2tm1Mtc/Hey2tm1Mtc
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
4a:
embryonic day 12.5
(more )
Specimen
4a:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: heart |
Present |
Regionally restricted |
4a
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Expression in coronary vasulature.
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Specimen
4b:
(close )
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Genetic Background:
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C.Cg-Hey2tm1Mtc
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Mutant Allele(s):
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Hey2tm1Mtc/Hey2tm1Mtc
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: heart |
Present |
Regionally restricted |
4b
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Expression in abnormal coronary vasulature.
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Specimen
4c:
embryonic day 15.5
(more )
Specimen
4c:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4d:
(close )
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Genetic Background:
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C.Cg-Hey2tm1Mtc
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Mutant Allele(s):
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Hey2tm1Mtc/Hey2tm1Mtc
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4e:
embryonic day 18.5
(more )
Specimen
4e:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4f:
(close )
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Genetic Background:
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C.Cg-Hey2tm1Mtc
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Mutant Allele(s):
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Hey2tm1Mtc/Hey2tm1Mtc
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: heart blood vessel |
Present |
Regionally restricted |
4f
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Expression in hyperplastic coronary veins.
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