Gene Expression Data
Assay Details
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Assay
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Reference:
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J:214712
Le Bras B, et al., In vivo assembly of the axon initial segment in motor neurons. Brain Struct Funct. 2014 Jul;219(4):1433-50
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5828213
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Gene symbol:
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Ank3
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Gene name:
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ankyrin 3, epithelial
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Results
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Specimen
6a1:
embryonic day 9.0
(more  )
Specimen
6a1:
(close  )
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Genetic Background:
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CRL:OF1
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6b1:
embryonic day 9.25
(more )
Note: Double labeled: green - Ank3; red - Nfasc (assay MGI:5828263).
Specimen
6b1:
(close )
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Genetic Background:
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CRL:OF1
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Age:
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embryonic day 9.25
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Ank3; red - Nfasc (assay MGI:5828263).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube lateral wall |
Present |
Regionally restricted |
6b1
,
6b3
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Expression is in a few motor neurons and along their axons extending out of the spinal cord.
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Specimen
6c1:
embryonic day 10.5
(more )
Note: Double labeled: green - Ank3; red - Nfasc (assay MGI:5828263).
Specimen
6c1:
(close )
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Genetic Background:
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CRL:OF1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Ank3; red - Nfasc (assay MGI:5828263).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube lateral wall |
Present |
Regionally restricted |
6c3
,
6c1
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Expression is in motor neurons and along their axons extending out of the spinal cord.
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Specimen
6d1:
embryonic day 11.5
(more )
Note: Double labeled: green - Ank3; red - Nfasc (assay MGI:5828263).
Specimen
6d1:
(close )
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Genetic Background:
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CRL:OF1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Ank3; red - Nfasc (assay MGI:5828263).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: neural tube lateral wall |
Present |
Regionally restricted |
6d3
,
6d1
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Expression is in the axons of motor neurons with a distal to proximal restriction.
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Specimen
6e1:
embryonic day 13.5
(more )
Note: Double labeled: green - Ank3; red - Nfasc (assay MGI:5828263).
Specimen
6e1:
(close )
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Genetic Background:
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CRL:OF1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Ank3; red - Nfasc (assay MGI:5828263).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: basal columns |
Present |
Regionally restricted |
6e3
,
6e1
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Expression is in the proximal axonal segments of motor neurons in the spinal cord.
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Specimen
6f1:
embryonic day 12.5
(more )
Specimen
6f1:
(close )
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Genetic Background:
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CRL:OF1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: basal columns |
Present |
Regionally restricted |
6f1
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Expression is in the axons of motor neurons with a distal to proximal restriction.
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Specimen
6f2:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Nfasctm1.1Brp/Nfasctm1.1Brp
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: basal columns |
Present |
Regionally restricted |
6f2
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Expression in this mutant is the same as in wild-type.
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Specimen
6g1:
embryonic day 13.5
(more )
Specimen
6g1:
(close )
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Genetic Background:
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CRL:OF1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: ventral grey horn |
Present |
Regionally restricted |
6g1
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Expression is in the proximal axonal segments of motor neurons in the spinal cord.
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Specimen
6g2:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Nfasctm1.1Brp/Nfasctm1.1Brp
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: ventral grey horn |
Present |
Regionally restricted |
6g2
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Expression in this mutant is the same as in wild-type.
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Specimen
S1b:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Nfasctm1.1Brp/Nfasctm1.1Brp
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S1d2:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Nfasctm1.1Brp/Nfasctm1.1Brp
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Ank3 (guinea pig antibody); blue - Ank3 (rabbit antibody; assay MGI:5828210); green - Ank3 (mouse antibody; assay MGI:5828294).
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Analysis Tools