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Gene Expression Data
Assay Details
Assay
Reference: J:169049 Varelas X, et al., The Crumbs complex couples cell density sensing to Hippo-dependent control of the TGF-beta-SMAD pathway. Dev Cell. 2010 Dec 14;19(6):831-44
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5828114
Gene symbol: Smad2
Gene name: SMAD family member 2
Antibody: Smad2/3 antibody (610842)
Detection system: Secondary antibody
Results
Specimen 2A 6 cells: embryonic day 2.5 (more )
Structure Level Pattern Image Note
TS3: 4-8 cell stage embryo Present 2A SMAD2/3 6 cells Expression is predominantly nuclear.
Specimen 2A Morula: embryonic day 3.0 (more )
Structure Level Pattern Image Note
TS3: compacted morula Present 2A SMAD2/3 Morula Expression is nuclear in the trophectoderm precursors.
Specimen 2A Blastocyst: embryonic day 3.5 (more )
Structure Level Pattern Image Note
TS4: inner cell mass Present 2A SMAD2/3 Blastocyst Diffuse expression in the cytoplasm.
TS4: trophectoderm Present 2A SMAD2/3 Blastocyst Expression is predominantly nuclear.
Specimen 2B: embryonic day 3.5 (more )
Structure Level Pattern Image Note
TS4: inner cell mass Present 2B SMAD2/3 , 2B merge Diffuse expression in the cytoplasm.
TS4: trophectoderm Present 2B merge , 2B SMAD2/3 Expression is predominantly nuclear.
Specimen 2C: embryonic day 5.5 (more )
Note: pre-gastrula stage embryo
Structure Level Pattern Image Note
TS8: extraembryonic visceral endoderm Strong 2C SMAD2 merge , 2C SMAD2 Expression is nuclear.
TS8: epiblast Present 2C SMAD2 merge , 2C SMAD2 Expression is cytoplasmic and nuclear.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory