Probe:	cTnt probe2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS10: embryo	Absent

Structure	Level	Pattern	Image	Note
TS11: lateral plate mesoderm	Present	Regionally restricted	3A/B	Expression specifically in lateral plate mesoderm in the region of the cardiogenic plate.
TS11: cardiogenic plate	Present		3A/B	Expression specifically in lateral plate mesoderm in the region of the cardiogenic plate.

Structure	Level	Pattern	Image	Note
TS17: heart	Strong
TS17: somite	Weak	Regionally restricted		Weak expression in a few of the most posterior somites.

Structure	Level	Pattern	Image	Note
TS20: heart	Strong		4A/B
TS20: somite	Weak	Regionally restricted	4A/B	Lower level of expression in posterior somites.
TS20: tail	Strong	Regionally restricted	4A/B	Strong expression in tip of tail.

Structure	Level	Pattern	Image	Note
TS20: trunk mesenchyme	Strong	Regionally restricted	4C/D	Strong expression in mesenchyme around cardinal veins and sinus venosus.
TS20: somite	Weak		4C/D

Structure	Level	Pattern	Image	Note
TS21: heart	Strong		5A/B
TS21: tongue	Strong		5A/B
TS21: crus of diaphragm	Weak		5A/B
TS21: urogenital sinus	Weak		5A/B
TS21: trunk musculature	Weak	Regionally restricted	5A/B	Weak but significant expression in muscles around vertebrae.
TS21: tail	Weak	Regionally restricted	5A/B	Weak but significant expression in tail muscle.

Structure	Level	Pattern	Image	Note
TS21: heart	Strong		5C/D
TS21: limb musculature	Weak		5C/D
TS21: trunk musculature	Weak		5C/D

Structure	Level	Pattern	Image	Note
TS24: bladder	Very strong		6A/B
TS24: heart	Very strong		6A/B
TS24: lower jaw	Weak		6A/B
TS24: tongue	Weak		6A/B

Structure	Level	Pattern	Image	Note
TS24: heart	Very strong		7A/B
TS24: limb musculature	Trace		7A/B
TS24: trunk musculature	Trace		7A/B