Antibody:	343-1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS21: nervous system	Present	Regionally restricted	5	Pattern of expression consistent with neuroepithelial and neuronal expression.
TS21: central nervous system nerve	Present		5
TS21: cerebral cortex intermediate zone	Present		5
TS21: peripheral nerve	Present		5
TS21: trigeminal nerve	Present		5
TS21: brainstem	Present	Regionally restricted	5	Expression in fiber tracts in brainstem and spinal cord.
TS21: spinal cord	Present	Regionally restricted	5	Expression in fiber tracts in brainstem and spinal cord.
TS21: skeletal muscle	Present		5
TS21: skin	Present		5
TS21: gut muscularis	Moderate	Regionally restricted	5	Moderate expression in smooth muscle of gut (muscularis externa).
TS21: cardiac muscle tissue	Weak		5
TS21: blood vessel	Absent		5
TS21: liver	Absent		5
TS21: lung	Absent		5

Structure	Level	Pattern	Image	Note
TS21: segmental spinal nerve	Present	Regionally restricted	6a	Expression in both peripheral and central roots of dorsal root ganglia.
TS21: dorsal root ganglion	Weak	Regionally restricted	6a	Less expression associated with cell bodies of dorsal root ganglion neurons.
TS21: rib pre-cartilage condensation	Present	Regionally restricted	6a	Expression in periosteum. No expression in chondrocytes.

Structure	Level	Pattern	Image	Note
TS21: telencephalon ventricular layer	Present	Regionally restricted	6b	Authors report significant expression surrounding ventricles in forebrain and hindbrain, appearing to outline neuroepithelial cells of ventricular zone, with highest levels along surface of ventricle.

Structure	Level	Pattern	Image	Note
TS21: pretubular aggregate	Present	Regionally restricted	9e	Expression localized to pretubular epithelial aggregates, some of which showing characteristic C-shape of condensing nephrons.
TS21: comma-shaped body	Present		9e	Expression localized to pretubular epithelial aggregates, some of which showing characteristic C-shape of condensing nephrons.
TS21: mesenchyme of metanephros	Weak	Regionally restricted	9e	Little expression in uninduced mesenchyme.
TS21: skeletal muscle	Present		9e