Foxa1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:16244 Ang SL, et al., The formation and maintenance of the definitive endoderm lineage in the mouse: involvement of HNF3/forkhead proteins. Development. 1993 Dec;119(4):1301-15
Assay type:	RNA in situ
MGI Accession ID:	MGI:5825120
Gene symbol:	Foxa1
Gene name:	forkhead box A1

Probe:	HNF3-alpha-cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Results

3C ©

Specimen 3C: embryonic day 7.0 (more

)
Note: Late primitive streak stage.

Structure	Level	Pattern	Image	Note
TS10: embryo endoderm	Present	Regionally restricted	3C	Expression was detected in embryonic endoderm throughout the posterior of the embryo.
TS10: embryo mesoderm	Absent		3C	No staining was detected in axial mesoderm.

3F ©

Specimen 3F: embryonic day 7.5 (more

)
Note: Headfold stage.

Structure	Level	Pattern	Image	Note
TS11: embryo endoderm	Present	Regionally restricted	3F	Expression was detected in the endoderm layer in the anterior midline.
TS11: neural plate	Absent		3F

3I ©

Specimen 3I: embryonic day 8.5 (more

)

Structure	Level	Image	Note
TS13: future midbrain	Present	3I
TS13: foregut diverticulum	Present	3I
TS13: midgut	Present	3I
TS13: notochord	Present	3I
TS13: neural tube floor plate	Absent	3I	No expression was detected in the ventral floorplate of the spinal cord.

3L ©

Specimen 3L: embryonic day 9.5 (more

)

Structure	Level	Image
TS15: hepatic primordium	Strong	3L
TS15: future hindbrain floor plate	Present	3L
TS15: neural tube floor plate	Present	3L

3O ©

Specimen 3O: embryonic day 16.5 (more

)

Structure	Level	Image
TS24: gut	Present	3O
TS24: liver	Present	3O
TS24: lung	Present	3O
TS24: pancreas	Present	3O
TS24: vertebral cartilage condensation	Absent	3O

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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