Gene Expression Data
Assay Details
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Assay
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Reference:
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J:16244
Ang SL, et al., The formation and maintenance of the definitive endoderm lineage in the mouse: involvement of HNF3/forkhead proteins. Development. 1993 Dec;119(4):1301-15
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5825080
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Gene symbol:
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Foxa2
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Gene name:
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forkhead box A2
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Probe:
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HNF3-beta-cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 6.5
(more  )
Note: Very early streak embryo.
Specimen
2A:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 6.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Very early streak embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: primitive streak |
Present |
Regionally restricted |
2A
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Expression was detected in a small patch of cells in the anterior part of the primitive streak.
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Specimen
2B:
embryonic day 7.0
(more )
Note: Midstreak embryo.
Specimen
2B:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 7.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Midstreak embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: primitive streak |
Present |
Regionally restricted |
2B
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The expression domain expanded to include a larger group of cells in the anterior third of the primitive streak.
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Specimen
2C:
embryonic day 7.0
(more )
Note: Late streak embryo.
Specimen
2C:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 7.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Late streak embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: node |
Present |
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2C
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Expression was found in both layers of the definitive node.
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| TS10: embryo endoderm |
Present |
Regionally restricted |
2C
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Expression was detected in anteriorly migrating mesoderm and endoderm cells of the head process.
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| TS10: embryo mesoderm |
Present |
Regionally restricted |
2C
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Expression was detected in anteriorly migrating mesoderm and endoderm cells of the head process.
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Specimen
2D:
embryonic day 7.5
(more )
Note: Headfold stage embryo.
Specimen
2D:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Headfold stage embryo.
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Specimen
2E:
embryonic day 7.5
(more )
Note: Late headfold/early foregut pocket stage embryo.
Specimen
2E:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Late headfold/early foregut pocket stage embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: foregut diverticulum |
Present |
Regionally restricted |
2E
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Expression was detected in medial and lateral regions of the foregut.
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| TS12: embryo endoderm |
Present |
Regionally restricted |
2E
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Endoderm expression became broader at the anterior end.
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Specimen
2F:
embryonic day 7.0
(more )
Note: Midstreak embryo.
Specimen
2F:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 7.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Midstreak embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo |
Present |
Regionally restricted |
2F
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Expression was detected in posterior epiblast.
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| TS10: embryo mesoderm |
Present |
Regionally restricted |
2F
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Expression was detected in delaminating mesoderm cells in the anterior streak.
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Specimen
4A:
embryonic day 8.5-9.0
(more )
Note: 8-9 somite embryo.
Specimen
4A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 8.5-9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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8-9 somite embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: neural tube |
Present |
Regionally restricted |
4A
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Expression was detected in ventral neural tube, from the forebrain to the posterior end, in a region encompassing the floorplate and cells lateral to it.
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| TS13: notochord |
Present |
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4A
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| TS13: gut |
Present |
Widespread |
4A
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Expression was detected in the entire gut. Expression was elevated in a portion of the gut beneath the cardiac region.
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| TS13: future prosencephalon |
Present |
Regionally restricted |
4A
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Expression extends more laterally than the floorplate.
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Specimen
4B:
embryonic day 8.5-9.0
(more )
Specimen
4B:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 8.5-9.0
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future prosencephalon |
Present |
Regionally restricted |
4B
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Expression extends more laterally than the floorplate.
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Specimen
4C:
embryonic day 9.0
(more )
Note: 15 somites.
Specimen
4C:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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15 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: hepatic primordium |
Strong |
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4C
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| TS14: gut |
Present |
Widespread |
4C
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Expression was detected in the entire gut. Expression was elevated in a portion of the gut beneath the cardiac region.
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