Antibody:	Rpt-9
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present		1A	Predominant nuclear expression.
TS17: 2nd branchial arch	Present		1A	Predominant nuclear expression.
TS17: brain	Present		1A	Predominant nuclear expression.
TS17: heart	Present		1A	Uniform distribution between nucleus and cytoplasm.

Structure	Level	Pattern	Image	Note
TS19: brain	Present		1B
TS19: branchial arch	Present		1B
TS19: heart	Present		1B
TS19: otocyst	Present	Regionally restricted	1B	Expression is in the otic placode.
TS19: eye	Present	Regionally restricted	1B	Expression is in the optic placode.

Structure	Level	Pattern	Image	Note
TS19: brain	Present		2A	Expression is predominantly nuclear but some cells have a perinuclear staining pattern.

Structure	Level	Pattern	Image	Note
TS20: brain	Strong		3A
TS20: heart	Strong		3A
TS20: mandibular process	Strong		3A
TS20: liver	Present		3A

Structure	Level	Pattern	Image	Note
TS21: Meckel's cartilage	Strong		3C
TS21: liver	Present		3C
TS21: brain	Weak		3C
TS21: heart	Weak		3C

Structure	Level	Pattern	Image	Note
TS19: liver	Weak	Uniform	4A	Expression is predominantly cytoplasmic and nuclear.

Structure	Level	Pattern	Image	Note
TS21: liver	Present		4B	Expression is in the cytoplasm and nuclei.

Structure	Level	Pattern	Image	Note
TS24: liver	Strong		4D	Expression is stronger in the nuclei.

Structure	Level	Pattern	Image	Note
TS21: lens	Present		4E

Structure	Level	Pattern	Image	Note
TS23: mandible	Present		4G
TS23: maxilla	Present		4G
TS23: head bone	Present		4G	Expression is in membranous facial bone.
TS23: tongue	Trace		4G

Structure	Level	Pattern	Image	Note
TS23: liver	Strong		5A
TS23: abdomen musculature	Present		5A
TS23: bone	Present		5A
TS23: hippocampus	Present		5A
TS23: lung	Present		5A
TS23: renal tubule	Present		5A
TS23: tooth	Present		5A
TS23: vertebra	Present		5A
TS23: head bone	Present	Regionally restricted	5A	Expression is in membranous facial bone.
TS23: pectoral girdle and thoracic body wall muscle	Present	Regionally restricted	5A	Expression is in thoracic muscle.
TS23: smooth muscle tissue	Absent		5A

Structure	Level	Pattern	Image	Note
TS23: liver	Strong		5B
TS23: abdomen musculature	Present		5B
TS23: lung	Present		5B
TS23: renal tubule	Present		5B
TS23: rib	Present		5B
TS23: vertebra	Present		5B
TS23: pectoral girdle and thoracic body wall muscle	Present	Regionally restricted	5B	Expression is in thoracic muscle.
TS23: smooth muscle tissue	Absent		5B

Structure	Level	Pattern	Image	Note
TS23: lobar bronchus epithelium	Present		5C
TS23: trachea epithelium	Absent		5C

Structure	Level	Pattern	Image	Note
TS23: axial muscle	Strong		5D
TS23: tongue	Weak		5D	Expression is nuclear.

Structure	Level	Pattern	Image	Note
TS24: bone	Strong		5E
TS24: epidermis	Strong		5E
TS24: liver	Strong		5E
TS24: embryo	Present	Regionally restricted	5E

Structure	Level	Pattern	Image	Note
TS24: epidermis	Present	Regionally restricted	5F	Expression is in all layers of the stratified dorsal epithelium.
TS24: dermis	Absent		5F

Structure	Level	Pattern	Image	Note
TS24: collecting duct	Present		5G
TS24: renal tubule	Present	Regionally restricted	5G	Expression is in epithelial cells.

Structure	Level	Pattern	Image	Note
TS24: adrenal cortex	Present		5H	Expression is uniform cytoplasmic and nuclear to strongly nuclear.

Structure	Level	Pattern	Image	Note
TS17: somite	Present			Uniform distribution between nucleus and cytoplasm.

Structure	Level	Pattern	Image	Note
TS22: Bowman's capsule of maturing renal corpuscle	Present

Structure	Level	Pattern	Image	Note
TS24: Bowman's capsule of maturing renal corpuscle	Present