Probe:	Perlecan cDNA1
Probe preparation:	labelled with digoxigenin DNA
Visualized with:	Alkaline phosphatase
Assay notes:	RNA in situ RT-PCR.

Structure	Level	Pattern	Image	Note
TS17: atrioventricular canal endocardial lining	Present		5A
TS17: bulboventricular groove	Present		5A

Structure	Level	Pattern	Image	Note
TS21: dorsal aorta	Strong		5B	Strong expression in the endothelial cells.

Structure	Level	Pattern	Image	Note
TS21: arterial blood vessel	Strong	Regionally restricted	5C	Strong expression in endothelial cells of major arterial branches in the mediastinal region.

Structure	Level	Pattern	Image	Note
TS21: artery	Strong	Regionally restricted	5E	Rostral aorta exhibited strong expression.
TS21: sympathetic ganglion	Strong	Regionally restricted	5E	Strong expression was detected in the mediastinal ganglia.
TS21: thymus primordium	Absent		5E

Structure	Level	Pattern	Image	Note
TS25: lesser omentum	Present		5F
TS25: stomach	Present	Regionally restricted	5F	Expression was detected below the lumen of the stomach. Intense expression was in the stroma.

Structure	Level	Pattern	Image	Note
TS21: hindgut	Present	Regionally restricted	5G	Expression was detected in the basolateral portions of the developing gastrointestinal tract. Adventitial cells showed positivity.
TS21: hindgut epithelium	Absent

Structure	Level	Pattern	Image	Note
TS21: dermis	Strong	Regionally restricted	5H	Expression was detected in the upper dermis.
TS21: skin	Present	Regionally restricted	5H	Expression was detected in the dermal-epidermal junction.
TS21: epidermis	Absent		5H

Structure	Level	Pattern	Image	Note
TS17: vertebral cartilage condensation	Present	Diffuse	6A

Structure	Level	Pattern	Image	Note
TS21: cartilage	Strong	Regionally restricted	6B	Expression was strong in cartilage mesenchyme in the developing facial structures.
TS21: face mesenchyme	Strong	Regionally restricted	6B	Expression was strong in cartilage mesenchyme in the developing facial structures.

Structure	Level	Pattern	Image	Note
TS21: rib cartilage condensation	Present	Regionally restricted	6C	Expression was concentrated in cells at the periphery of the cartilaginous nodules of the ribs.
TS21: perichondrium	Present		6C

Structure	Level	Pattern	Image	Note
TS25: cartilage	Present	Regionally restricted	6D	A strong pocket of expression predominantly at the periphery of the cartilage in the developing head of the femur.
TS25: perichondrium	Present		6D
TS25: connective tissue	Present	Regionally restricted	6D	Expression was detected in the regions of ligaments just proximal to their attachment.

Structure	Level	Pattern	Image	Note
TS25: rib cartilage	Present	Regionally restricted	6F	Expression was detected at the periphery of the cartilaginous structure.

Structure	Level	Pattern	Image	Note
TS25: hindlimb	Present		6G
TS25: hindlimb dermis	Present	Regionally restricted	6G	Expression was detected in the upper dermis.