Probe:	STC probe1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: mesonephros mesenchyme	Weak	Regionally restricted	1A	Weak expression in undifferentiated mesenchyme surrounding mesonephric tubules.

Structure	Level	Pattern	Image	Note
TS19: mesenchyme of metanephros	Present	Regionally restricted		Expression in mesenchyme cells on the rostral and caudal tips of the emerging metanephric kidney.
TS19: mesonephric tubule	Absent			No expression in tubular epithelial cells of the degenerating mesonephros.
TS19: nephric duct, mesonephric portion	Absent
TS19: urogenital ridge	Present			Expression in the rostral aspect of the mesonephros within the area corresponding to the genital ridge.

Structure	Level	Pattern	Image	Note
TS20: nephrogenic interstitium	Present		1C	Expression in cells on the perimeter of the metanephric kidney, corresponding to the metanephrogenic blastema.
TS20: nephric duct, mesonephric portion	Present		1C

Structure	Level	Pattern	Image	Note
TS21: genitourinary system	Present	Regionally restricted		Authors state that the pattern of expression seen at E12.5 persisted to E13.5.

Structure	Level	Pattern	Image	Note
TS22: nephrogenic interstitium	Weak			Level of expression in metanephrogenic blastemal cells declined markedly.
TS22: collecting duct	Present	Regionally restricted		Expression most evident in ureteric bud epithelium, the future collecting duct system.

Structure	Level	Pattern	Image	Note
TS23: collecting duct	Present	Widespread	1F	Expression exclusively associated with derivatives of the ureteric bud (collecting ducts) throughout cortical and medullary kidney.

Structure	Level	Pattern	Image	Note
TS25: inner medullary collecting duct	Strong		2A
TS25: outer medullary collecting duct	Strong		2A
TS25: ureter	Present	Regionally restricted	2A	Expression in ureter most abundant in condensing mesenchymal cells immediately surrounding the ureter epithelium.

Structure	Level	Pattern	Image	Note
TS26: collecting duct	Present	Widespread	2C	Expression throughout all branches of the collecting duct system.
TS26: glomerular tuft	Ambiguous		2C	Authors report little evidence of expression in glomeruli and proximal nephron segments.
TS26: nephron	Ambiguous		2C	Authors report little evidence of expression in glomeruli and proximal nephron segments.

Structure	Level	Pattern	Image	Note
TS26: ureter	Present	Regionally restricted		Highest expression in mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS20: testis	Present	Regionally restricted	2E	In the early E12 testis, expression in mesenchymal cells surrounding the emerging sex cords.

Structure	Level	Pattern	Image	Note
TS20: interstitium of the testis	Present		2F	In the late E12 testis, expression concentrated in the interstitial mesenchyme cells.
TS20: primary sex cord	Absent		2F

Structure	Level	Pattern	Image	Note
TS20: ovary	Absent

Structure	Level	Pattern	Image	Note
TS22: interstitium of the testis	Present		2G

Structure	Level	Pattern	Image	Note
TS25: testis	Present	Regionally restricted	3A	Consolidation of the highest expressing cells around the perimeter of the seminiferous tubules.
TS25: ductus deferens	Moderate		3A

Structure	Level	Pattern	Image	Note
TS20: nephric duct, mesonephric portion	Present	Regionally restricted	3E	Expression concentrated in mesenchyme surrounding the mesonephric ducts.

Structure	Level	Pattern	Image	Note
TS20: nephric duct, mesonephric portion	Strong	Regionally restricted	3E inset	Strong expression at both rostral and caudal poles.

Structure	Level	Pattern	Image	Note
TS25: ovary	Present		3G
TS25: paramesonephric duct	Present	Regionally restricted	3G	Expression in the developing Mullerian ducts confined to ductal epithelial cells.