Gene Expression Data
Assay Details
Assay
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Reference:
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J:226028
Lewandowski JP, et al., Spatiotemporal regulation of GLI target genes in the mammalian limb bud. Dev Biol. 2015 Oct 1;406(1):92-103
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5823697
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Gene symbol:
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Ptch2
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Gene name:
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patched 2
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Probe:
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Ptch2 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A - Ptch2 - E10.5:
embryonic day 10.5
(more )
Specimen
2A - Ptch2 - E10.5:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud |
Present |
Regionally restricted |
2A Ptch2 - E10.5
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This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.
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Specimen
2A - Ptch2 - E11.5:
embryonic day 11.5
(more )
Specimen
2A - Ptch2 - E11.5:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 11.5
|
Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: forelimb bud |
Present |
Regionally restricted |
2A Ptch2 - E11.5
|
This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.
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Specimen
S1A - Ptch2 - E10.5:
embryonic day 10.5
(more )
Specimen
S1A - Ptch2 - E10.5:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud |
Present |
Regionally restricted |
S1A Ptch2 - E10.5
|
This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.
|
Specimen
S1A - Ptch2 - E11.5:
embryonic day 11.5
(more )
Specimen
S1A - Ptch2 - E11.5:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: forelimb bud |
Present |
Regionally restricted |
S1A Ptch2 - E11.5
|
This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.
|
Specimen
Supplemental Table 1 - Ptch2:
embryonic day 10.5
(more )
Specimen
Supplemental Table 1 - Ptch2:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud |
Strong |
Regionally restricted |
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Staining is observed in the posterior limb bud.
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Specimen
Ptch2_b25_E11.5a_RF_8NOV13:
embryonic day 11.5
(more )
Specimen
Ptch2_b25_E11.5a_RF_8NOV13:
(close )
Genetic Background:
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Swiss Webster
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Age:
|
embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
Ptch2_b25_E11.5a_8NOV13:
embryonic day 11.5
(more )
Specimen
Ptch2_b25_E11.5a_8NOV13:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 11.5
|
Sex:
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Not Specified
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Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
|