Probe:	pOtd9
Probe preparation:	Double stranded labelled with digoxigenin RNA
Assay notes:	Specimens were hybridized with probes for two different markers: Otx2 and T (assay MGI:5823401).

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		1A
TS15: future midbrain	Present		1A

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		1B	A hypomorphic mutation of Nodal (D600) has no phenotypic consequence in the heterozygote.
TS15: future midbrain	Present		1B	A hypomorphic mutation of Nodal (D600) has no phenotypic consequence in the heterozygote.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		1C	Embryos heterozygous for a null allele of Nodal have no phenotype.
TS15: future midbrain	Present		1C	Embryos heterozygous for a null allele of Nodal have no phenotype.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		1D	Embryos heterozygous for the severe loss of function kumba (Ku) allele of Zic2 have no discernible phenotype.
TS15: future midbrain	Present		1D	Embryos heterozygous for the severe loss of function kumba (Ku) allele of Zic2 have no discernible phenotype.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Ambiguous		1E	Embryos homozygous for the severe loss of function kumba (Ku) allele of Zic2 exhibit forebrain dysmorphology that can include a mild loss of telencephalic tissue.
TS15: future midbrain	Present		1E	Embryos homozygous for this allele never exhibit loss of diencephalon or mesencephalon.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		1F	Embryos homozygous for the D600 allele of Nodal exhibit a spectrum of defects, including no phenotype.
TS15: future midbrain	Present		1F	Embryos homozygous for the D600 allele of Nodal exhibit a spectrum of defects, including no phenotype.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Absent		1G	When homozygous, a hypomorphic mutations of Nodal (D600) can cause moderate truncation in which some of the midbrain is retained.
TS15: future midbrain	Present		1G	When homozygous, a hypomorphic mutations of Nodal (D600) can cause moderate truncation in which some of the midbrain is retained.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Absent		1H	When homozygous, a hypomorphic mutations of Nodal (D600) can cause severe truncation I which all forebrain and midbrain tissue is absent.
TS15: future midbrain	Absent		1H	When homozygous, a hypomorphic mutations of Nodal (D600) can cause severe truncation I which all forebrain and midbrain tissue is absent.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Ambiguous		1I	Decreasing Nodal function on the Zic2 Ku background leads to moderate or severe truncation.
TS15: future midbrain	Ambiguous		1I	Decreasing Nodal function on the Zic2 Ku background leads to moderate or severe truncation.