Gene Expression Data
Assay Details
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Assay
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Reference:
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J:238567
Houtmeyers R, et al., Zic2 mutation causes holoprosencephaly via disruption of NODAL signalling. Hum Mol Genet. 2016 Sep 15;25(18):3946-3959
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5823401
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Gene symbol:
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T
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Gene name:
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brachyury, T-box transcription factor T
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Probe:
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brachyury probe2
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Probe preparation:
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Double stranded labelled with digoxigenin RNA
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Assay notes:
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Specimens were hybridized with probes for two different markers: T and Otx2 (assay MGI:5823402).
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Results
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Specimen
1A:
embryonic day 9.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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involves: 129/SvEv
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Present |
Regionally restricted |
1A
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Expression in the primitive streak.
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| TS15: notochord |
Present |
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1A
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Specimen
1B:
embryonic day 9.5; Nodaltm2Rob/Nodal +
(more )
Specimen
1B:
(close )
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Genetic Background:
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129S(Cg)-Nodaltm2Rob
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Mutant Allele(s):
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Nodaltm2Rob/Nodal+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Present |
Regionally restricted |
1B
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Expression in the primitive streak.
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| TS15: notochord |
Present |
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1B
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Specimen
1C:
embryonic day 9.5; Nodaltm1Rob/Nodal +
(more )
Specimen
1C:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Present |
Regionally restricted |
1C
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Expression in the primitive streak.
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| TS15: notochord |
Present |
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1C
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Specimen
1D:
embryonic day 9.5; Zic2Ku/Zic2 +
(more )
Specimen
1D:
(close )
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Genetic Background:
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129S.CAnNCrl(C3)-Zic2Ku
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Mutant Allele(s):
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Zic2Ku/Zic2+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Present |
Regionally restricted |
1D
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Expression in the primitive streak.
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| TS15: notochord |
Present |
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1D
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Specimen
1E:
(close )
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Genetic Background:
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129S.CAnNCrl(C3)-Zic2Ku
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Mutant Allele(s):
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Zic2Ku/Zic2Ku
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Present |
Regionally restricted |
1E
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Expression in the primitive streak.
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| TS15: notochord |
Present |
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1E
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Specimen
1F:
(close )
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Genetic Background:
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129S(Cg)-Nodaltm2Rob
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Mutant Allele(s):
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Nodaltm2Rob/Nodaltm2Rob
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Present |
Regionally restricted |
1F
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Expression in the primitive streak.
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| TS15: notochord |
Present |
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1F
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Specimen
1G:
(close )
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Genetic Background:
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129S(Cg)-Nodaltm2Rob
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Mutant Allele(s):
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Nodaltm2Rob/Nodaltm2Rob
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Present |
Regionally restricted |
1G
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Expression in the primitive streak.
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| TS15: notochord |
Present |
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1G
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Specimen
1H:
(close )
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Genetic Background:
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129S(Cg)-Nodaltm2Rob
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Mutant Allele(s):
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Nodaltm2Rob/Nodaltm2Rob
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Present |
Regionally restricted |
1H
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Expression in the primitive streak.
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| TS15: notochord |
Ambiguous |
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1H
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Authors report loss of anterior mesendodoerm.
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Specimen
1I:
(close )
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Genetic Background:
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129S.Cg-Nodaltm1Rob Zic2Ku
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
Zic2Ku/Zic2Ku
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Present |
Regionally restricted |
1I
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Expression in the primitive streak.
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| TS15: notochord |
Ambiguous |
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1I
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Authors report loss of anterior mesendodoerm.
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Analysis Tools
