Antibody:	Anti-RNF20 (ab32629)
Detection system:	Secondary antibody coupled to Alexa Fluor 488
Assay notes:	Two antibodies were described for this protein: MGI:5823270 and MGI:5823273. The one used in this assay was not specified.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Ubiquitous	1A

Structure	Level	Pattern	Image	Note
TS17: forebrain ventricular layer	Present	Non-Uniform	1B Bre1a	A few cells in the ventricular zone of the dorsal and ventral forebrain were immunolabeled much more weakly than in the surrounding cells.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Ubiquitous	1C Bre1a

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Ubiquitous	1D Bre1a	Co-localization with Tubb3, an immature neuronal marker, was noted in cells near to the basal surface of dorsal forebrain.

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Ubiquitous	1E
TS20: cerebral cortex ventricular layer	Present	Non-Uniform	1F Bre1a , 1E
TS20: forebrain ventricular layer	Present	Non-Uniform	1E , 1G Bre1a	A few cells in the ventricular zone of the dorsoventral boundary were immunolabeled much more weakly than in the surrounding cells.
TS20: medial ganglionic eminence	Present	Non-Uniform	1E , 1H Bre1a	A few cells in the ventricular zone of the medial ganglionic eminence were immunolabeled much more weakly than in the surrounding cells.

Structure	Level	Pattern	Image	Note
TS20: forebrain ventricular layer	Present	Non-Uniform	1I Bre1a	A few cells in the ventricular zone of the forebrain were immunolabeled much more weakly than in the surrounding cells.

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Ubiquitous	1J bottom

Structure	Level	Pattern	Image	Note
TS24: forebrain	Present	Ubiquitous	1K

Structure	Level	Pattern	Image	Note
TS27: forebrain	Present	Ubiquitous	1L