Probe:	Pde3a cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	Two probes were described. Fig. 2 did not specify whether the coding sequence probe MGI:5819076 or the 3' untranslated region probe MGI:5819077 was used. The 3' untranslated region probe was used in Fig. S3.

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Regionally restricted	2d 12.5 d	Expression was detected in domains involved in digit formation. Expression was consistent with a role in chondrogenesis.

Structure	Level	Pattern	Image	Note
TS21: forelimb	Present	Regionally restricted	2d 13.5 d	Expression was detected in domains involved in digit formation. Expression was consistent with a role in chondrogenesis.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Absent		S3a 11.5d

Structure	Level	Pattern	Image	Note
TS20: forelimb	Weak		S3a 12.5d	Light expression was noted. Expression was consistent with a role in chondrogenesis.

Structure	Level	Pattern	Image	Note
TS21: forelimb	Weak		S3a 13.5d	Light expression was noted. Expression was consistent with a role in chondrogenesis.