Gene Expression Data
Assay Details
Assay
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Reference:
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J:14524
Clauss IM, et al., In situ hybridization studies suggest a role for the basic region-leucine zipper protein hXBP-1 in exocrine gland and skeletal development during mouse embryogenesis. Dev Dyn. 1993 Jun;197(2):146-56
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5816634
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Gene symbol:
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Timp1
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Gene name:
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tissue inhibitor of metalloproteinase 1
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Probe:
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TIMP probe1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Assay notes:
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Specimens were embedded in paraffin or processed for cryosectioning; authors did not specify which.
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Results
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Specimen
day 10.5 not shown:
embryonic day 10.5
(more )
Specimen
day 10.5 not shown:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Specimen
3A:
embryonic day 11.5
(more )
Specimen
3A:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Specimen
3B/C:
embryonic day 13.5
(more )
Specimen
3B/C:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS21: skeleton |
Present |
Regionally restricted |
3B/C
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Expression in periosteal cuff of bone forming the diaphysis. Expression principally in chondroblasts of mature zone of growth cartilage. Much lower levels of expression in chondroblasts than in osteoblasts.
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Specimen
3E:
embryonic day 14.5
(more )
Specimen
3E:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Specimen
3F/G:
embryonic day 18.5
(more )
Specimen
3F/G:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS26: femur |
Strong |
Regionally restricted |
3F/G
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High levels of expression in periosteal derived osteoblasts of bony diaphysis, and in osteoblasts participating in endochondral ossification. No expression in hypertrophic and degenerative cartilage cells.
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Specimen
3H:
embryonic day 16.5
(more )
Specimen
3H:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS24: maxilla |
Present |
Regionally restricted |
3H
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Expression in the fine cancellous bone of maxilla. Expression persists in more mature osteoblasts. Most intense expression in preosteoblasts one or several cell layers from the osteoblasts of newly formed bones.
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