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Gene Expression Data
Assay Details
Assay
Reference: J:99651 Hartwig S, et al., Glypican-3 modulates inhibitory Bmp2-Smad signaling to control renal development in vivo. Mech Dev. 2005 Jul-Aug;122(7-8):928-38
Assay type: Western blot
MGI Accession ID: MGI:5816253
Gene symbol: Smad1
Gene name: SMAD family member 1
Antibody: anti-phospho-SMAD1
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes: Authors describe two glypican-3 monoclonal antibodies; MGI:5816244 may have been used.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Genetic BackgroundMutant Allele(s)Sex
Wt E13.5 TS21: metanephros Present involves: C57BL/6 Not Specified
Bmp2+/- E13.5 TS21: metanephros Present involves: 129S7/SvEvBrd * C57BL/6 Bmp2tm1Brd/Bmp2+Not Specified
Gpc3+/- E13.5 TS21: metanephros Weak (a) involves: C57BL/6 Gpc3tm1Fil/Gpc3+Female
Gpc3+/-;Bmp2+/- E13.5 TS21: metanephros Weak (b) involves: 129S7/SvEvBrd * C57BL/6 Bmp2tm1Brd/Bmp2+, Gpc3tm1Fil/Gpc3+Female
Notes:
(a) Expression level decreased 1.12-fold compared to wild type.
(b) Expression level decreased 1.38-fold compared to wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory