Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45678
Henderson DJ, et al., Over-expression of the chondroitin sulphate proteoglycan versican is associated with defective neural crest migration in the Pax3 mutant mouse (splotch). Mech Dev. 1997 Dec;69(1-2):39-51
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5811770
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Gene symbol:
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Pax1
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Gene name:
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paired box 1
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Probe:
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Pax1 probe26
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
E9.5 wt not shown:
embryonic day 9.5
(more  )
Specimen
E9.5 wt not shown:
(close  )
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Genetic Background:
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involves: 101 * C3H/He * CBA/Ca
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: sclerotome |
Present |
Widespread |
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Expression throughout sclerotome from the time of its formation.
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Specimen
E9.5 Sp2H not shown:
(close )
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Genetic Background:
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involves: 101 * C3H/He * CBA/Ca
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Mutant Allele(s):
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Pax3Sp-2H/Pax3Sp-2H
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: sclerotome |
Present |
Widespread |
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No major difference in expression between Sp2H homozygotes and wild type littermates.
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Specimen
8A wt:
embryonic day 10.5
(more )
Specimen
8A wt:
(close )
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Genetic Background:
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involves: 101 * C3H/He * CBA/Ca
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
8A Sp2H:
(close )
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Genetic Background:
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involves: 101 * C3H/He * CBA/Ca
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Mutant Allele(s):
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Pax3Sp-2H/Pax3Sp-2H
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: branchial pouch |
Present |
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8A Sp2H
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Expression in somites along the length of the body and in branchial pouches in a closely similar distribution compared to wild type embryo.
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| TS17: somite |
Present |
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8A Sp2H
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Expression in somites along the length of the body and in branchial pouches in a closely similar distribution compared to wild type embryo.
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Specimen
8B:
embryonic day 10.5
(more )
Note: Specimens were embedded in a mixture of 0.45% gelatine, 27% albumen, 18% glucose in PBS, solidified by addition of 1/10 volume of 30% glutaraldehyde.
Specimen
8B:
(close )
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Genetic Background:
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involves: 101 * C3H/He * CBA/Ca
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Embedding:
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Gelatin
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Note:
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Specimens were embedded in a mixture of 0.45% gelatine, 27% albumen, 18% glucose in PBS, solidified by addition of 1/10 volume of 30% glutaraldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: sclerotome |
Present |
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8B
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Expression confined to sclerotome.
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Specimen
8C:
embryonic day 10.5; Pax3Sp-2H/ Pax3Sp-2H
(more )
Note: Specimens were embedded in a mixture of 0.45% gelatine, 27% albumen, 18% glucose in PBS, solidified by addition of 1/10 volume of 30% glutaraldehyde.
Specimen
8C:
(close )
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Genetic Background:
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involves: 101 * C3H/He * CBA/Ca
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Mutant Allele(s):
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Pax3Sp-2H/Pax3Sp-2H
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Embedding:
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Gelatin
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Note:
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Specimens were embedded in a mixture of 0.45% gelatine, 27% albumen, 18% glucose in PBS, solidified by addition of 1/10 volume of 30% glutaraldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: sclerotome |
Present |
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8C
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Expression confined to sclerotome with no marked difference compared to wild type embryos.
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Specimen
E11.5 wt not shown:
embryonic day 11.5
(more )
Specimen
E11.5 wt not shown:
(close )
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Genetic Background:
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involves: 101 * C3H/He * CBA/Ca
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: sclerotome |
Present |
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Expression throughout sclerotome.
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Specimen
E11.5 Sp2H not shown:
(close )
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Genetic Background:
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involves: 101 * C3H/He * CBA/Ca
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Mutant Allele(s):
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Pax3Sp-2H/Pax3Sp-2H
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: sclerotome |
Present |
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No major difference in expression between sp2H homozygotes and wild type littermates.
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Analysis Tools