Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45678
Henderson DJ, et al., Over-expression of the chondroitin sulphate proteoglycan versican is associated with defective neural crest migration in the Pax3 mutant mouse (splotch). Mech Dev. 1997 Dec;69(1-2):39-51
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5811768
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Gene symbol:
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Pax3
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Gene name:
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paired box 3
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Probe:
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Pax3 probe39
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
E9.0 wt not shown:
embryonic day 9.0
(more  )
Specimen
E9.0 wt not shown:
(close  )
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Genetic Background:
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involves: 101 * C3H/He * CBA/Ca
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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Not Specified
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: somite |
Weak |
Regionally restricted |
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Low levels of expression in somitic mesoderm.
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Specimen
E9.5 wt not shown:
embryonic day 9.5
(more )
Specimen
E9.5 wt not shown:
(close )
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Genetic Background:
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involves: 101 * C3H/He * CBA/Ca
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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Not Specified
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: dermomyotome |
Present |
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Expression restricted to dermomyotome.
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| TS15: sclerotome |
Absent |
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Expression is down-regulated in the sclerotome.
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Specimen
6A:
embryonic day 10.5
(more )
Note: Specimens were embedded in a mixture of 0.45% gelatine, 27% albumen, 18% glucose in PBS, solidified by addition of 1/10 volume of 30% glutaraldehyde.
Specimen
6A:
(close )
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Genetic Background:
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involves: 101 * C3H/He * CBA/Ca
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Embedding:
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Gelatin
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Note:
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Specimens were embedded in a mixture of 0.45% gelatine, 27% albumen, 18% glucose in PBS, solidified by addition of 1/10 volume of 30% glutaraldehyde.
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Specimen
6C:
embryonic day 10.5; Pax3Sp-2H/ Pax3Sp-2H
(more )
Note: Specimens were embedded in a mixture of 0.45% gelatine, 27% albumen, 18% glucose in PBS, solidified by addition of 1/10 volume of 30% glutaraldehyde.
Specimen
6C:
(close )
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Genetic Background:
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involves: 101 * C3H/He * CBA/Ca
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Mutant Allele(s):
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Pax3Sp-2H/Pax3Sp-2H
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Embedding:
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Gelatin
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Note:
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Specimens were embedded in a mixture of 0.45% gelatine, 27% albumen, 18% glucose in PBS, solidified by addition of 1/10 volume of 30% glutaraldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Present |
Regionally restricted |
6C
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Authors state that truncated transcripts present in similar distribution to wild type in dorsal neural tube.
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| TS17: dermomyotome |
Weak |
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6C
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Expression diminished in dermomyotome.
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| TS17: limb bud |
Absent |
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6C
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Specimen
7A:
embryonic day 11.5
(more )
Note: Specimens were embedded in a mixture of 0.45% gelatine, 27% albumen, 18% glucose in PBS, solidified by addition of 1/10 volume of 30% glutaraldehyde.
Specimen
7A:
(close )
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Genetic Background:
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involves: 101 * C3H/He * CBA/Ca
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Embedding:
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Gelatin
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Note:
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Specimens were embedded in a mixture of 0.45% gelatine, 27% albumen, 18% glucose in PBS, solidified by addition of 1/10 volume of 30% glutaraldehyde.
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Specimen
7C:
embryonic day 11.5; Pax3Sp-2H/ Pax3Sp-2H
(more )
Note: Specimens were embedded in a mixture of 0.45% gelatine, 27% albumen, 18% glucose in PBS, solidified by addition of 1/10 volume of 30% glutaraldehyde.
Specimen
7C:
(close )
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Genetic Background:
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involves: 101 * C3H/He * CBA/Ca
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Mutant Allele(s):
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Pax3Sp-2H/Pax3Sp-2H
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Embedding:
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Gelatin
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Note:
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Specimens were embedded in a mixture of 0.45% gelatine, 27% albumen, 18% glucose in PBS, solidified by addition of 1/10 volume of 30% glutaraldehyde.
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Analysis Tools