Probe:	Otx3 probe2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present
TS13: optic placode	Present

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Strong		2A1	From 9.5 to 11.5 dpc, expression is craniocaudally delimited to rostral region of developing nervous system. Anterior and posterior boundaries of expression coincide with those of the diencephalon and mesencephalon, respectively.
TS15: future midbrain	Strong		2A1	From 9.5 to 11.5 dpc, expression is craniocaudally delimited to rostral region of developing nervous system. Anterior and posterior boundaries of expression coincide with those of the diencephalon and mesencephalon, respectively.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	2A2	Beginning from 10.5 dpc, expression progressively declines in anterior region.
TS17: midbrain	Present	Regionally restricted	2A2	Beginning from 10.5 dpc, expression progressively declines in anterior region.
TS17: midbrain-hindbrain junction	Strong		2A2	Most intense expression coincides with midbrain-hindbrain boundary.
TS17: eye	Present		2A2

Structure	Level	Pattern	Image	Note
TS19: eye	Present		2A3
TS19: diencephalon	Present		2A3
TS19: metencephalon	Present		2A3	Expression gradually enhanced in a craniocaudal direction within mesencephalon, metencephalon and myelencephalon.
TS19: midbrain	Present		2A3	Expression gradually enhanced in a craniocaudal direction within mesencephalon, metencephalon and myelencephalon.
TS19: myelencephalon	Present		2A3	Expression gradually enhanced in a craniocaudal direction within mesencephalon, metencephalon and myelencephalon.