Probe:	Igsf8 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Whole-mount specimens were postfixed in 4% PFA/0.2% glutaraldehyde.

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	3A	Expression is as a longitudinal stripe that runs from just caudal to the otic vesicle to the tailbud.

Structure	Level	Pattern	Image	Note
TS15: 3rd branchial arch	Present		3B
TS15: neural tube	Absent		3B	At the level of the heart expression is absent in the neural tube.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3C	Expression is in the ventral one-third of the neural tube.
TS15: neural tube floor plate	Absent		3C

Structure	Level	Pattern	Image	Note
TS15: tail unsegmented mesenchyme	Very strong		3D
TS15: neural tube	Weak	Regionally restricted	3D	Expression is in the posterior neuropore.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Regionally restricted	3E	Expression is as a longitudinal stripe that runs from just caudal to the otic vesicle to the tailbud.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Regionally restricted	3F	Expression is as two domains on either side of the midline.

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Very strong		3G

Structure	Level	Pattern	Image	Note
TS17: gut epithelium	Present	Regionally restricted	3H	Expression is in the dorsal epithelium in a discrete region of the gut.

Structure	Level	Pattern	Image	Note
TS17: 3rd branchial arch ectoderm	Present		3I
TS17: 3rd branchial arch mesenchyme	Present		3I

Structure	Level	Pattern	Image	Note
TS17: neural tube	Very strong	Regionally restricted	3J	Expression is in the ventral one-third and is absent in the region of differentiating motor neurons.
TS17: gut epithelium	Present	Regionally restricted	3J	Expression is in the dorsal epithelium in a discrete region of the gut.
TS17: neural tube floor plate	Absent		3J

Structure	Level	Pattern	Image	Note
TS17: neural tube	Very strong	Regionally restricted	3K	Expression is in the ventral one-third.
TS17: neural tube floor plate	Absent		3K

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3L	Expression is in a dorsal and ventral longitudinal stripe.
TS17: brain	Present		3L
TS17: tail mesenchyme	Absent		3L

Structure	Level	Pattern	Image	Note
TS17: telencephalon ventricular layer	Present	Regionally restricted	3M	Punctate expression at the lateral edge of the ventricular zone.

Structure	Level	Pattern	Image	Note
TS17: hindbrain ventricular layer	Very strong	Regionally restricted	3N	Expression is at the lateral edge in the dorsal half.
TS17: hindbrain roof plate	Very strong		3N

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	3O	Expression in the ventricular zone of the ventral neural tube at forelimb level. Expression is stronger in the dorsal half.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	3P	In this caudal section expression in the dorsal neural tube is weaker, but intense expression remains present in the ventral one-third of the ventricular zone.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3Q	Expression is weak dorsal and is stronger in the ventral neural ventricular zone of the neural tube at hindlimb level.

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present		3R
TS15: tail unsegmented mesenchyme	Present		3R
TS15: neural tube	Absent		3R

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present		3S
TS15: neural tube	Absent		3S

Structure	Level	Pattern	Image	Note
TS15: neural tube	Absent		3T

Structure	Level	Pattern	Image	Note
TS15: tail unsegmented mesenchyme	Present		3U
TS15: tail neural tube	Absent		3U

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present		3V
TS17: neural tube	Weak		3V

Structure	Level	Pattern	Image	Note
TS17: gut	Present		3W

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present		3X
TS17: hindbrain ventricular layer	Strong	Regionally restricted	3X	Expression is stronger at the lateral edge.
TS17: neural tube	Weak	Regionally restricted	3X

Structure	Level	Pattern	Image	Note
TS17: gut	Present		3Y
TS17: neural tube	Weak		3Y

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	4A	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	4E	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	4I	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		4K	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS15: gut	Present	Regionally restricted	5A	Expression is in a discrete domain corresponding to the developing pancreas.

Structure	Level	Pattern	Image	Note
TS15: gut epithelium	Present	Regionally restricted	5C	Expression is in the dorsal gut epithelium corresponding to the developing pancreas.

Structure	Level	Pattern	Image	Note
TS17: gut	Present	Regionally restricted	5E	Expression is in a discrete domain.

Structure	Level	Pattern	Image	Note
TS17: gut	Present	Regionally restricted	5G	Expression is in a discrete domain.

Structure	Level	Pattern	Image	Note
TS19: diencephalon ventricular layer	Present		5I	Expression is is most intense at the lateral edge of the ventricular zone, with less intense expression within the ventricular zone itself.
TS19: hindbrain ventricular layer	Present		5I	Expression is is most intense at the lateral edge of the ventricular zone, with less intense expression within the ventricular zone itself.
TS19: telencephalon ventricular layer	Present		5I	Expression is is most intense at the lateral edge of the ventricular zone, with less intense expression within the ventricular zone itself.

Structure	Level	Pattern	Image	Note
TS19: neural tube ventricular layer	Strong		5J	Expression is stronger at the lateral edge at forelimb bud level.
TS19: neural tube roof plate	Strong		5J

Structure	Level	Pattern	Image	Note
TS19: bronchus epithelium	Present		5K
TS19: esophagus epithelium	Present		5K

Structure	Level	Pattern	Image	Note
TS20: atrium cardiac muscle	Present		5L
TS20: bronchus epithelium	Present		5L
TS20: ventricle cardiac muscle	Present		5L

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present		5M
TS20: large intestine	Present	Regionally restricted	5M	Expression is in the epithelium of the rectum.
TS20: urinary system	Present	Regionally restricted	5M	Expression is in the epithelium of the bladder.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	Present		5N	Expression is is most intense at the lateral edge of the ventricular zone, with less intense expression within the ventricular zone itself.

Structure	Level	Pattern	Image	Note
TS21: hindbrain ventricular layer	Present		5O	Expression is is most intense at the lateral edge of the ventricular zone, with less intense expression within the ventricular zone itself.
TS21: vestibulo-cochlear ganglion	Present		5O

Structure	Level	Pattern	Image	Note
TS21: lung epithelium	Present		5P

Structure	Level	Pattern	Image	Note
TS23: telencephalon ventricular layer	Strong		5Q
TS23: cerebral cortex intermediate zone	Present		5Q
TS23: cortical plate	Present		5Q

Structure	Level	Pattern	Image	Note
TS23: choroid plexus	Present		5R

Structure	Level	Pattern	Image	Note
TS23: neural retina	Present		5S

Structure	Level	Pattern	Image	Note
TS23: olfactory epithelium	Present		5T

Structure	Level	Pattern	Image	Note
TS23: vomeronasal organ	Present		5U

Structure	Level	Pattern	Image	Note
TS25: rectum epithelium	Present		5V
TS25: reproductive system epithelium	Present		5V
TS25: urethra epithelium	Present		5V

Structure	Level	Pattern	Image	Note
TS25: gut epithelium	Present		5W

Structure	Level	Pattern	Image	Note
TS25: dorsal root ganglion	Present		5X
TS25: metanephros	Present		5X

Structure	Level	Pattern	Image	Note
TS20: pancreas	Absent